These mice carry a knock-out mutation of the Plk2 gene. Homozygotes exhibit a decrease in soluble alpha synuclein phosphorylation in the brain and may be useful in studies of Parkinson's Disease.
Dr. Winston Thomas, Elan Pharmaceuticals
Mice that are homozygous for the targeted mutation and on the 129S background are viable and fertile, the Donating Investigator reports. On the C57Bl/6NTac background, few live homozygotes are observed. No gene product (mRNA) is detected by qRT-PCR analysis of primary
cortical cultures. Alpha synuclein phosphorylation is decreased by approximately 70% in cortical cultures from homozygous embryos, when compared to wildtype controls. In one 2-3 month old homozygous mouse, a similar decrease in soluble alpha synuclein phosphorylation was observed.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a NEO cassette was used to disrupt all 14 exons of the Plk2, polo-like kinase 2, gene. The construct was electroporated into unspecified C57BL/6NTac derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6NTac mice. The mice were then backcrossed to 129S6/SvEvTac using a speed congenic protocol, for approximately 6 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to 129S1/SvImJ (Stock No. 002448) at least once to establish the colony.
|Allele Name||targeted mutation 1, Elan Pharmaceuticals Inc|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Plk2, polo like kinase 2|
|Strain of Origin||C57BL/6NTac|
|Molecular Note||All 14 exons were replaced with a neo cassette. The absence of transcript expression was confirmed by quantitative RT-PCR on cortical cultures.|
When maintaining a live colony on the 129S background, these mice can be bred as homozygotes.
When using the Plk2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #017001 in your Materials and Methods section.