STOCK Tg(SNCA*E46K)3Elan/J

Stock number: 017000
Product name: BAC-Tg3(SNCA^E46K)
Research areas: Neurobiology Research

Description

These transgenic mice express the mutant human SNCA, synuclein, alpha (non A4 component of amyloid precursor), with the E46K mutation in exon 3. Hemizygous mice display age dependent synaptic degeneration in the striatum. This line may be a useful model for studies of Parkinson's Disease.

Detailed description

These transgenic mice express the mutant human SNCA, synuclein, alpha (non A4 component of amyloid precursor), with the E46K mutation in exon 3. Mice hemizygous for the transgenic insert are viable, fertile, and normal in size. Transgenic mice accumulate alpha synuclein and develop age-dependent dopaminergic (TH) fiber degeneration. Hemizygous mice display the loss of striatal tyrosine hydroxylase positive fibers by 6 months of age. The Donating Investigator recommends avoiding crossing to C57BL/6J because the Nnt deletion may have an effect on phenotype. This allele is also available on a congenic C57BL/6NJ background, which does not carrying the Nnt, see stock number 18768.

Development

The human bacterial artificial chromosome (BAC) RP11-458H10 containing the entire SNCA, synuclein, alpha (non A4 component of amyloid precursor), gene, was modified to include the E46K mutation in exon 3. The modified BAC was microinjected into the pronucleus of B6SJLF2 fertilized eggs. The resulting transgenic mice (from founder line 3) were subsequently backcrossed to B6D2F1/Tac for 10 generations prior to arrival at The Jackson Laboratory. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.

Allele

Symbol: Tg(SNCA*E46K)3Elan
Name: transgene insertion 3, Elan Pharmaceuticals Inc
MGI accession ID: MGI:5431984
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(SNCA*E46K)3Elan
Marker name: transgene insertion 3, Elan Pharmaceuticals Inc
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: SNCA,synuclein alpha,human
Molecular note: The human bacterial artificial chromosome (BAC) RP11-458H10 containing the entire SNCA, synuclein, alpha (non A4 component of amyloid precursor), gene, was modified to include the E46K mutation in exon 3. Line 3 was generated.