These transgenic mice express the mutant human SNCA, synuclein, alpha (non A4 component of amyloid precursor), with the E46K mutation in exon 3. Hemizygous mice display age dependent synaptic degeneration in the striatum. This line may be a useful model for studies of Parkinson's Disease.
Dr. Winston Thomas, Elan Pharmaceuticals
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These transgenic mice express the mutant human SNCA, synuclein, alpha (non A4 component of amyloid precursor), with the E46K mutation in exon 3. Mice hemizygous for the transgenic insert are viable, fertile, and normal in size. Transgenic mice accumulate alpha synuclein and develop age-dependent dopaminergic (TH) fiber degeneration. Hemizygous mice display the loss of striatal tyrosine hydroxylase positive fibers by 6 months of age. The Donating Investigator recommends avoiding crossing to C57BL/6J because the Nnt deletion may have an effect on phenotype. This allele is also available on a congenic C57BL/6NJ background, which does not carrying the Nnt, see
stock number 18768.
The human bacterial artificial chromosome (BAC) RP11-458H10 containing the entire SNCA, synuclein, alpha (non A4 component of amyloid precursor), gene, was modified to include the E46K mutation in exon 3. The modified BAC was microinjected into the pronucleus of B6SJLF2 fertilized eggs. The resulting transgenic mice (from founder line 3) were subsequently backcrossed to B6D2F1/Tac for 10 generations prior to arrival at The Jackson Laboratory. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.
Expressed Gene | SNCA, synuclein alpha, human |
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Site of Expression |
Allele Name | transgene insertion 3, Elan Pharmaceuticals Inc |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | BAC-Tg3(SNCAsupE46K/sup) |
Gene Symbol and Name | Tg(SNCA*E46K)3Elan, transgene insertion 3, Elan Pharmaceuticals Inc |
Gene Synonym(s) | |
Promoter | SNCA, synuclein alpha, human |
Expressed Gene | SNCA, synuclein alpha, human |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | The human bacterial artificial chromosome (BAC) RP11-458H10 containing the entire SNCA, synuclein, alpha (non A4 component of amyloid precursor), gene, was modified to include the E46K mutation in exon 3. Line 3 was generated. |
Mutations Made By | Dr. Winston Thomas, Elan Pharmaceuticals |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator recommends avoiding crossing to C57BL/6J because the Nnt deletion may have an effect on phenotype.
When using the BAC-Tg3(SNCAE46K) mouse strain in a publication, please cite the originating article(s) and include JAX stock #017000 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(SNCA*E46K)3Elan |
Frozen Mouse Embryo | STOCK Tg(SNCA*E46K)3Elan/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(SNCA*E46K)3Elan/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(SNCA*E46K)3Elan/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(SNCA*E46K)3Elan/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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