B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J

Stock number: 016997
Research areas: Neurobiology Research, Research Tools

Description

These Axin2-rtTA transgenic mice express the optimized reverse tetracycline-controlled transactivator (rtTA2^S-M2) protein directed by the mouse Axin2 promoter. These mice are a Tet-On tool that allows conditional, dox-inducible expression of genes in Axin2 expressing cells and may be useful for studying craniofacial, skeletal and early neural development.

Detailed description

These transgenic mice express an optimized reverse tetracycline-controlled transactivator, rtTAS*M2, or rtTA2^S-M2, under the control of the mouse Axin2 expression cassette (which contains 2883 nucleotides upstream of exon 1, exon 1, intron 1 and part of exon 2). Transgene expression mimics the endogenous Axin2 expression pattern, and is detected in dorsal neural tube neuroepithelial cells, migrating neural crest cells, and branchial arch post-migratory neural crest cells. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.

Development

A transgenic construct containing an optimized reverse tetracycline-controlled transactivator, rtTAS*M2, or rtTA2^S-M2, under the control of the mouse Axin2 expression cassette (which contains 2883 nucleotides upstream of exon 1, exon 1, intron 1 and part of exon 2) was injected into fertilized B6CBAF2 mouse eggs. Founder line 7 was subsequently established. The mice were then backcrossed to C57BL/6J for 4 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Axin2-rtTA2S*M2)7Cos
Name: transgene insertion 7, Frank Costantini
MGI accession ID: MGI:5292557
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Axin2-rtTA2S*M2)7Cos
Marker name: transgene insertion 7, Frank Costantini
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F2
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: expression is detected in dorsal neural tube neuroepithelial cells, migrating neural crest cells, and branchial arch post-migratory neural crest cells.
Molecular note: The transgene construct contains an optimized reverse tetracycline-controlled transactivator, rtTAS*M2, or rtTA2S-M2, under the control of the mouse Axin2 expression cassette (which contains 2883 nucleotides upstream of exon 1, exon 1, intron 1 and part of exon 2). Founder line 7 was established.