These Axin2-rtTA transgenic mice express the optimized reverse tetracycline-controlled transactivator (rtTA2S-M2) protein directed by the mouse Axin2 promoter. These mice are a Tet-On tool that allows conditional, dox-inducible expression of genes in Axin2 expressing cells and may be useful for studying craniofacial, skeletal and early neural development.
Frank Costantini, Columbia University Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
These transgenic mice express an optimized reverse tetracycline-controlled transactivator, rtTAS*M2, or rtTA2S-M2, under the control of the mouse Axin2 expression cassette (which contains 2883 nucleotides upstream of exon 1, exon 1, intron 1 and part of exon 2). Transgene expression mimics the endogenous Axin2 expression pattern, and is detected in dorsal neural tube neuroepithelial cells, migrating neural crest cells, and branchial arch post-migratory neural crest cells. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
A transgenic construct containing an optimized reverse tetracycline-controlled transactivator, rtTAS*M2, or rtTA2S-M2, under the control of the mouse Axin2 expression cassette (which contains 2883 nucleotides upstream of exon 1, exon 1, intron 1 and part of exon 2) was injected into fertilized B6CBAF2 mouse eggs. Founder line 7 was subsequently established. The mice were then backcrossed to C57BL/6J for 4 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
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Site of Expression | expression is detected in dorsal neural tube neuroepithelial cells, migrating neural crest cells, and branchial arch post-migratory neural crest cells. |
Allele Name | transgene insertion 7, Frank Costantini |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | Axin2-rtTA |
Gene Symbol and Name | Tg(Axin2-rtTA2S*M2)7Cos, transgene insertion 7, Frank Costantini |
Gene Synonym(s) | |
Promoter | Axin2, axin 2, mouse, laboratory |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | expression is detected in dorsal neural tube neuroepithelial cells, migrating neural crest cells, and branchial arch post-migratory neural crest cells. |
Strain of Origin | (C57BL/6 x CBA)F2 |
Chromosome | UN |
Molecular Note | The transgene construct contains an optimized reverse tetracycline-controlled transactivator, rtTAS*M2, or rtTA2S-M2, under the control of the mouse Axin2 expression cassette (which contains 2883 nucleotides upstream of exon 1, exon 1, intron 1 and part of exon 2). Founder line 7 was established. |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make this strain homozygous.
When using the B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016997 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Axin2-rtTA2S*M2)7Cos |
Frozen Mouse Embryo | B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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