A congenic version of this strain is available as Stock No. 028862.
Vgat-ires-Cre knock-in mice have Cre recombinase expression directed to inhibitory GABAergic neuron cell bodies, without disrupting endogenous vesicular inhibitory amino acid transporter expression. These mice may be useful for removing floxed sequences in studies of GABAergic neuronal function.
Of note, the same Vgat-ires-Cre knock-in allele is also available on a C57BL/6J genetic background as Stock No. 028862.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Slc32a1 | solute carrier family 32 (GABA vesicular transporter), member 1 |
Slc32a1tm2(cre)Lowl knock-in mice (commonly referred to as Vgat-ires-Cre mice) have Cre recombinase expression directed to inhibitory GABAergic neuron cell bodies, without disrupting endogenous vesicular inhibitory amino acid transporter expression. Both heterozygous and homozygous mice are viable and fertile with no gross physical abnormalities. Specifically, Cre recombinase activity is detected in inhibitory GABAergic neuron cell bodies in the caudate putamen, suprachiasmatic nucleus, central amygdaloid nucleus, and zona incerta, nucleus accumbens, lateral septum, medial septum, reticular nucleus of the thalamus, substantia nigra pars reticulata, and Purkinje cell layer of the cerebellum.
When crossed with a strain containing loxP site-flanked sequences, Cre-mediated recombination results in tissue-specific deletion of flanked sequences in the offspring.
Of note, the targeted Slc32a1 gene is located ~3.5 kbp (~1.9 cM) from the nonagouti locus (A). These C57BL/6;FVB;129S6 Vgat-ires-Cre knock-in mice have agouti coat color; presumably due to the presence of 129S6-derived Aw allele (from the ES cells used in creating the Vgat-ires-Cre allele).
A targeting vector was used to insert an IRES-Cre cassette downstream of the stop codon of the solute carrier family 32 (GABA vesicular transporter), member 1 gene (Slc32a1 or VGAT) on chromosome 2. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then crossed to FVB. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase activity is detected in inhibitory GABAergic neuron cell bodies in the caudate putamen, suprachiasmatic nucleus, central amygdaloid nucleus, and zona incerta, nucleus accumbens, lateral septum, medial septum, reticular nucleus of the thalamus, substantia nigra pars reticulata, and Purkinje cell layer of the cerebellum. |
Allele Name | targeted mutation 2, Bradford B Lowell |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | vGATiresCre; Vgat-ires-Cre |
Gene Symbol and Name | Slc32a1, solute carrier family 32 (GABA vesicular transporter), member 1 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase activity is detected in inhibitory GABAergic neuron cell bodies in the caudate putamen, suprachiasmatic nucleus, central amygdaloid nucleus, and zona incerta, nucleus accumbens, lateral septum, medial septum, reticular nucleus of the thalamus, substantia nigra pars reticulata, and Purkinje cell layer of the cerebellum. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | A targeting vector, constructed from a BAC clone containing the Slc32a1 (Vgat) gene, was utilized to insert an internal ribosomal entry site followed by the cre recombinase sequence into the Slc32a1 locus just downstream of the endogenous stop codon. Cre is expressed from the Slc32a1 gene promoter. |
Mutations Made By | Dr. Jia Yu, Beth Israel Deaconess Medical Center |
Both heterozygous and homozygous mice are viable and fertile with no gross physical abnormalities. When maintaining a live colony, homozygous mice may be bred together.
When using the Vgat-ires-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #016962 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Slc32a1<tm2(cre)Lowl> |
Frozen Mouse Embryo | STOCK Slc32a1<tm2(cre)Lowl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Slc32a1<tm2(cre)Lowl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Slc32a1<tm2(cre)Lowl>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Slc32a1<tm2(cre)Lowl>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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