Overview

Also Known As: Vgat-ires-cre

A congenic version of this strain is available as Stock No. 028862.

Vgat-ires-Cre knock-in mice have Cre recombinase expression directed to inhibitory GABAergic neuron cell bodies, without disrupting endogenous vesicular inhibitory amino acid transporter expression. These mice may be useful for removing floxed sequences in studies of GABAergic neuronal function.
Of note, the same Vgat-ires-Cre knock-in allele is also available on a C57BL/6J genetic background as Stock No. 028862.

Donating Investigator

Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)

Genetic overview

Genetic Background	Generation

Slc32a1^tm2(cre)Lowl

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Slc32a1	solute carrier family 32 (GABA vesicular transporter), member 1

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Research Applications

Research Tools
Neurobiology Research
Cancer Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Slc32a1^tm2(cre)Lowl knock-in mice (commonly referred to as Vgat-ires-Cre mice) have Cre recombinase expression directed to inhibitory GABAergic neuron cell bodies, without disrupting endogenous vesicular inhibitory amino acid transporter expression. Both heterozygous and homozygous mice are viable and fertile with no gross physical abnormalities. Specifically, Cre recombinase activity is detected in inhibitory GABAergic neuron cell bodies in the caudate putamen, suprachiasmatic nucleus, central amygdaloid nucleus, and zona incerta, nucleus accumbens, lateral septum, medial septum, reticular nucleus of the thalamus, substantia nigra pars reticulata, and Purkinje cell layer of the cerebellum.
When crossed with a strain containing loxP site-flanked sequences, Cre-mediated recombination results in tissue-specific deletion of flanked sequences in the offspring.

Of note, the targeted Slc32a1 gene is located ~3.5 kbp (~1.9 cM) from the nonagouti locus (A). These C57BL/6;FVB;129S6 Vgat-ires-Cre knock-in mice have agouti coat color; presumably due to the presence of 129S6-derived A^w allele (from the ES cells used in creating the Vgat-ires-Cre allele).

Development

A targeting vector was used to insert an IRES-Cre cassette downstream of the stop codon of the solute carrier family 32 (GABA vesicular transporter), member 1 gene (Slc32a1 or VGAT) on chromosome 2. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then crossed to FVB. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is detected in inhibitory GABAergic neuron cell bodies in the caudate putamen, suprachiasmatic nucleus, central amygdaloid nucleus, and zona incerta, nucleus accumbens, lateral septum, medial septum, reticular nucleus of the thalamus, substantia nigra pars reticulata, and Purkinje cell layer of the cerebellum.

Control Suggestions

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Vong L; Ye C; Yang Z; Choi B; Chua S Jr; Lowell BB. 2011. Leptin Action on GABAergic Neurons Prevents Obesity and Reduces Inhibitory Tone to POMC Neurons. Neuron 71(1):142-54PubMed: 21745644 MGI: J:174691

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Genetics

Slc32a1^tm2(cre)Lowl

Allele Symbol: Slc32a1^tm2(cre)Lowl

Allele Name	targeted mutation 2, Bradford B Lowell
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	targeted mutation 2, Bradford B Lowell; Slc32a1^tm2(cre)Lowl
Gene Symbol and Name	Slc32a1, solute carrier family 32 (GABA vesicular transporter), member 1
Gene Synonym(s)	expressed sequence R75019; R75019; VIAAT; VGAT; R75019; Viaat; vesicular inhibitory amino acid transporter; Vgat; Viaat
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is detected in inhibitory GABAergic neuron cell bodies in the caudate putamen, suprachiasmatic nucleus, central amygdaloid nucleus, and zona incerta, nucleus accumbens, lateral septum, medial septum, reticular nucleus of the thalamus, substantia nigra pars reticulata, and Purkinje cell layer of the cerebellum.
Strain of Origin	129S6/SvEvTac
Chromosome	2
Molecular Note	A targeting vector, constructed from a BAC clone containing the Slc32a1 (Vgat) gene, was utilized to insert an internal ribosomal entry site followed by the cre recombinase sequence into the Slc32a1 locus just downstream of the endogenous stop codon. Cre is expressed from the Slc32a1 gene promoter.
Mutations Made By	Dr. Jia Yu, Beth Israel Deaconess Medical Center

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Developmental Biology Research
  - Cre-lox System
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
- Cancer Research
  - Cre-lox System
- Cre-lox System
  - Cre Recombinase Expression
- Cardiovascular Research
  - Cre-lox System
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue
Cancer Research
- Cre-lox System
  - Cre recombinase expression

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Slc32a1^tm2(cre)Lowl/Slc32a1⁺
involves: 129S6/SvEvTac

normal phenotype

no abnormal phenotype detected
- Normal - heterozygotes do not display a detectable abnormal phenotype

References

Vong L; Ye C; Yang Z; Choi B; Chua S Jr; Lowell BB. 2011. Leptin Action on GABAergic Neurons Prevents Obesity and Reduces Inhibitory Tone to POMC Neurons. Neuron 71(1):142-54PubMed: 21745644 MGI: J:174691

Additional References

Liao GY; Kinney CE; An JJ; Xu B. 2019. TrkB-expressing neurons in the dorsomedial hypothalamus are necessary and sufficient to suppress homeostatic feeding. Proc Natl Acad Sci U S A 116(8):3256-3261PubMed: 30718415 MGI: J:272924

Additional - Slc32a1^tm2(cre)Lowl related

Technical Support

Contact Technical Support

Genotyping Protocols
- Separated PCR: Slc32a1^tm2(cre)Lowl
- Genotyping resources and troubleshooting
Breeding Considerations

Both heterozygous and homozygous mice are viable and fertile with no gross physical abnormalities. When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the Vgat-ires-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #016962 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Slc32a1<tm2(cre)Lowl>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Also Known As: Vgat-ires-cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Slc32a1tm2(cre)Lowl

Allele Symbol: Slc32a1tm2(cre)Lowl

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Slc32a1tm2(cre)Lowl/Slc32a1+involves: 129S6/SvEvTac

normal phenotype

References

Additional References

Additional - Slc32a1tm2(cre)Lowl related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Slc32a1^tm2(cre)Lowl

Allele Symbol: Slc32a1^tm2(cre)Lowl

Genotype: Slc32a1^tm2(cre)Lowl/Slc32a1⁺
involves: 129S6/SvEvTac

Additional - Slc32a1^tm2(cre)Lowl related