mThy1-hSNCA mice contain a transgene consisting of the mouse thymus cell antigen 1 (Thy-1) promoter directing expression of the human α-synuclein (SNCA) gene. These mice may be useful for studying the Parkinson's disease pathogenesis and neurodegeneration elicited by the toxic effects of aggregation and somatic accumulation of SNCA.
Kuldip Dave, The Michael J. Fox Foundation
Genetic Background | Generation |
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Allele Type |
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Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
mThy1-hSNCA transgenic mice have the mouse thymus cell antigen 1 (Thy-1) promoter directing expression of the human α-synuclein (SNCA) gene. The transgene integrated into chromosome 14 causing a duplication in Slc4a7 (solute carrier family 4, sodium bicarbonate cotransporter, member 7). The duplication results in a functional knock-out of Slc4a7 in homozygous mice. Hemizygotes are viable, fertile, and normal in size. This line carries ~23 copies of the transgene; expression is relatively high in the cerebral cortex and hippocampus, and relatively low in the kidneys. These mice may be useful for studying the Parkinson's disease pathogenesis and neurodegeneration elicited by the toxic effects of aggregation and somatic accumulation of SNCA.
There is a different founder line with the same construct available,
stock number 17682, which has about 2-fold higher expression of the transgenic protein in the striatum and cortex.
The mThy1-hSNCA transgene consists of the human α-synuclein (SNCA) gene driven by a mouse thymus cell antigen 1 (Thy-1) promoter. The construct was microinjected into fertilized C57BL/6NTac oocytes. The transgene integrated into chromosome 14 causing a duplication in Slc4a7 (solute carrier family 4, sodium bicarbonate cotransporter, member 7). Males from founder line 12 were bred to C57BL/6NTac females to establish the colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.
Expressed Gene | SNCA, synuclein alpha, human |
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Site of Expression |
Allele Name | transgene insertion 12, The Michael J Fox Foundation |
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Allele Type | Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | mThy1-hSNCA; Tg(Thy1-SNCA)12Youy |
Gene Symbol and Name | Slc4a7, solute carrier family 4, sodium bicarbonate cotransporter, member 7 |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | SNCA, synuclein alpha, human |
Strain of Origin | C57BL/6NTac |
Chromosome | 14 |
Molecular Note | The transgene contains the human alpha-synuclein (SNCA) gene driven by a mouse thymus cell antigen 1 (Thy-1) promoter. Line 12 inserted into the gene at 14719103-14719104 (Build GRCm38/mm10) resulting in a duplication. The duplication results in a functional knock-out of Slc4a7 in homozygous mice. |
Mutations Made By | Yingbin Ouyang, Taconic |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6NJ inbred mice (Stock No. 005304).
When using the mThy1-hSNCA, line 12 mouse strain in a publication, please cite the originating article(s) and include JAX stock #016936 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Thy1-SNCA)12Youy |
Frozen Mouse Embryo | C57BL/6N-Slc4a7<Tg(Thy1-SNCA)12Mjff>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6N-Slc4a7<Tg(Thy1-SNCA)12Mjff>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6N-Slc4a7<Tg(Thy1-SNCA)12Mjff>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6N-Slc4a7<Tg(Thy1-SNCA)12Mjff>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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