mThy1-hSNCA mice contain a transgene consisting of the mouse thymus cell antigen 1 (Thy-1) promoter directing expression of the human α-synuclein (SNCA) gene. These mice may be useful for studying the Parkinson's disease pathogenesis and neurodegeneration elicited by the toxic effects of aggregation and somatic accumulation of SNCA.
Kuldip Dave, The Michael J. Fox Foundation
mThy1-hSNCA transgenic mice have the mouse thymus cell antigen 1 (Thy-1) promoter directing expression of the human α-synuclein (SNCA) gene. The transgene integrated into chromosome 14 causing a duplication in Slc4a7 (solute carrier family 4, sodium bicarbonate cotransporter, member 7). The duplication results in a functional knock-out of Slc4a7 in homozygous mice. Hemizygotes are viable, fertile, and normal in size. This line carries ~23 copies of the transgene; expression is relatively high in the cerebral cortex and hippocampus, and relatively low in the kidneys. These mice may be useful for studying the Parkinson's disease pathogenesis and neurodegeneration elicited by the toxic effects of aggregation and somatic accumulation of SNCA.
There is a different founder line with the same construct available,
stock number 17682, which has about 2-fold higher expression of the transgenic protein in the striatum and cortex.
The mThy1-hSNCA transgene consists of the human α-synuclein (SNCA) gene driven by a mouse thymus cell antigen 1 (Thy-1) promoter. The construct was microinjected into fertilized C57BL/6NTac oocytes. The transgene integrated into chromosome 14 causing a duplication in Slc4a7 (solute carrier family 4, sodium bicarbonate cotransporter, member 7). Males from founder line 12 were bred to C57BL/6NTac females to establish the colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.
|Expressed Gene||SNCA, synuclein alpha, human|
|Site of Expression|
|Allele Name||transgene insertion 12, The Michael J Fox Foundation|
|Allele Type||Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||mThy1-hSNCA; Tg(Thy1-SNCA)12Youy|
|Gene Symbol and Name||Slc4a7, solute carrier family 4, sodium bicarbonate cotransporter, member 7|
|Promoter||Thy1, thymus cell antigen 1, theta, mouse, laboratory|
|Expressed Gene||SNCA, synuclein alpha, human|
|Strain of Origin||C57BL/6NTac|
|Molecular Note||The transgene contains the human alpha-synuclein (SNCA) gene driven by a mouse thymus cell antigen 1 (Thy-1) promoter. Line 12 inserted into the gene at 14719103-14719104 (Build GRCm38/mm10) resulting in a duplication. The duplication results in a functional knock-out of Slc4a7 in homozygous mice.|
|Mutations Made By|| |
Yingbin Ouyang, Taconic
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6NJ inbred mice (Stock No. 005304).
When using the mThy1-hSNCA, line 12 mouse strain in a publication, please cite the originating article(s) and include JAX stock #016936 in your Materials and Methods section.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.