B6.129P2(Cg)-Cdh1^tm1Cle/J

Stock number: 016933
Product name: E-cadherin-mCFP
Research areas: Cell Biology Research, Research Tools

Description

E-cadherin-mCFP mice have monomeric cyan fluorescent protein (mCFP) fused to the endogenous cadherin 1 protein (Cdh1). These mice are useful for imaging studies in all types of epithelia that express E-cadherin, including crypt cells of the small intestine.

Detailed description

These Cdh1 (cadherin 1; also called E-cadherin) targeted mutant mice express a monomeric cyan fluorescent protein (mCFP) fused to the endogenous E-cadherin protein. The cDNA of mCFP is targeted to replace the STOP codon in exon 16 of Cdh1, preserving the native 3' UTR. This mouse line enables the visualization of all epithelial structures within the mouse. Three dimensional (3D) crypt architecture in the small intestine has been visualized to depths of 125 um. Appropriate expression has also been seen in hair follicles, skin, lung, stomach and colon. The E-cadherin-mCFP fusion protein correctly localizes in the plasma membrane of cells. The fusion strategy results in one extra valine as a linker between E-cadherin and mCFP. These mice are likely to be useful for imaging studies in all types of epithelia that express E-cadherin.

Development

Monomeric cyan fluorescent protein (mCFP) and a loxP-flanked neomycin cassette were targeted to exon 16 (encoding the endogenous STOP codon) to maintain normal expression levels and patterns. The 129P2/OlaHsd-derived IB10/E14IB10 embryonic stem cell line was used to create the mutation. Resultant mice were crossed with a PGK-cre mouse of unknown genetic background to excise the neomycin cassette. This strain was backcrossed to C57BL/6 for 4-6 generations by the donating laboratory.

Allele

Symbol: Cdh1^tm1Cle
Name: targeted mutation 1, Hans Clevers
MGI accession ID: MGI:4838590
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Cdh1
Marker name: cadherin 1
Chromosome: 8
Strain of origin: 129P2/OlaHsd
Expressed genes: CFP,Cyan Fluorescent Protein,jellyfish; Cdh1,cadherin 1,mouse, laboratory
Site of expression: Expression is seen in all types of epithelia that express E-cadherin, including crypt cells of the small intestine.
Molecular note: Monomeric cyan fluorescent protein (mCFP) and a loxP-flanked neomycin cassette were targeted to exon 16 (encoding the endogenous STOP codon) to maintain normal expression levels and patterns.