STOCK Tg(Myh2-DsRed2)1Jrs/J

Stock number: 016921
Research areas: Research Tools

Description

These MyIIA-RFP transgenic mice express DsRed2 Fluorescent Protein specifically in Type IIA muscle fibers. This strain may be used to visualize Type IIA muscle fibers, and for studies of muscle development, architecture, plasticity and innervation.

Detailed description

These transgenic mice express the DsRed2 Fluorescent Protein under the control of the mouse Myh2 (myosin, heavy polypeptide 2, skeletal muscle, adult) promoter. Fluorescence is specific to Type IIA skeletal muscle fibers throughout the body. More than 90% of fluorescent fibers co-label with Type IIA MyHC antibodies, and more than 90% of fibers that are IIA-positive by immunohistochemistry are DsRed-positive. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice may be used to visualize Type IIA muscle fibers in whole mounts or in live animals as well as in fixed or unfixed sections; currently available antibodies can be used only in unfixed sections.

Development

An approximately 100kb mouse bacterial artificial chromosome (BAC) containing the entire mouse Myh2 (myosin, heavy polypeptide 2, skeletal muscle, adult) gene, was modified by inserting Red Fluorescent Protein DsRed2 coding sequence in the translation start site. DNA from the correctly modified BAC was used to generate the transgene, which was microinjected into fertilized CBA X C57BL/6 mouse eggs. Founder line 1 was subsequently established. The resulting male transgenic mice were crossed to CD1 mice for 14 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Myh2-DsRed2)1Jrs
Name: transgene insertion 1, Joshua R Sanes
MGI accession ID: MGI:5291634
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Myh2-DsRed2)1Jrs
Marker name: transgene insertion 1, Joshua R Sanes
Chromosome: UN
Strain of origin: CBA x C57BL/6
Expressed genes: RFP,Red Fluorescent Protein,coral
Site of expression: DsRed labels all Type IIA skeletal muscle fibers.
Molecular note: An approximately 100kb mouse bacterial artificial chromosome (BAC) containing the entire mouse Myh2 (myosin, heavy polypeptide 2, skeletal muscle, adult) gene, was modified by inserting Red Fluorescent Protein DsRed2 coding sequence in the translation start site. Line 1 was generated.