These MyIIA-RFP transgenic mice express DsRed2 Fluorescent Protein specifically in Type IIA muscle fibers. This strain may be used to visualize Type IIA muscle fibers, and for studies of muscle development, architecture, plasticity and innervation.
Joshua R Sanes, Harvard University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These transgenic mice express the DsRed2 Fluorescent Protein under the control of the mouse Myh2 (myosin, heavy polypeptide 2, skeletal muscle, adult) promoter. Fluorescence is specific to Type IIA skeletal muscle fibers throughout the body. More than 90% of fluorescent fibers co-label with Type IIA MyHC antibodies, and more than 90% of fibers that are IIA-positive by immunohistochemistry are DsRed-positive. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice may be used to visualize Type IIA muscle fibers in whole mounts or in live animals as well as in fixed or unfixed sections; currently available antibodies can be used only in unfixed sections.
An approximately 100kb mouse bacterial artificial chromosome (BAC) containing the entire mouse Myh2 (myosin, heavy polypeptide 2, skeletal muscle, adult) gene, was modified by inserting Red Fluorescent Protein DsRed2 coding sequence in the translation start site. DNA from the correctly modified BAC was used to generate the transgene, which was microinjected into fertilized CBA X C57BL/6 mouse eggs. Founder line 1 was subsequently established. The resulting male transgenic mice were crossed to CD1 mice for 14 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression | DsRed labels all Type IIA skeletal muscle fibers. |
Allele Name | transgene insertion 1, Joshua R Sanes |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | MyIIA-RFP |
Gene Symbol and Name | Tg(Myh2-DsRed2)1Jrs, transgene insertion 1, Joshua R Sanes |
Gene Synonym(s) | |
Promoter | Myh2, myosin, heavy polypeptide 2, skeletal muscle, adult, mouse, laboratory |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | DsRed labels all Type IIA skeletal muscle fibers. |
Strain of Origin | CBA x C57BL/6 |
Chromosome | UN |
Molecular Note | An approximately 100kb mouse bacterial artificial chromosome (BAC) containing the entire mouse Myh2 (myosin, heavy polypeptide 2, skeletal muscle, adult) gene, was modified by inserting Red Fluorescent Protein DsRed2 coding sequence in the translation start site. Line 1 was generated. |
Mutations Made By | Joshua Sanes, Harvard University |
When maintaining a live colony, these mice can be bred as hemizygotes.
When using the STOCK Tg(Myh2-DsRed2)1Jrs/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016921 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier r for Tg(Myh2-DsRed2)1Jrs |
Frozen Mouse Embryo | STOCK Tg(Myh2-DsRed2)1Jrs/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Myh2-DsRed2)1Jrs/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Myh2-DsRed2)1Jrs/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Myh2-DsRed2)1Jrs/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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