This diabetes resistant congenic NOD.Itgb2 deficient mutant may be useful for in vivo analysis of leukocyte integrin-dependent adhesion in inflammatory disease models, specifically Type 1 Diabetes.
Dr. Christopher G. Kevil, LSU Health Sciences Center
Commonly called NOD.CD18, mice homozygous for the Itgb2tm2Bay targeted mutation are viable and fertile. Homozygous females are poor breeders, often producing 1 litter prior to becoming non-productive. Examination, of uterine horns, indicates poor healing post partum (unpublished communication).
Unlike C57BL/6 mutant (Stock No. 003329) mice, congenic NOD Itgb2 mutant mice do not develop dermatitis or facial submandibular erosions. Similar to the C57BL/6 mutant, the congenic NOD Itgb2 mutant displays splenomegaly and lymphadenopathy. Splenic cells of NOD mutant mice exhibit significantly increased levels of CD29 and significantly decreased levels of Cd62L and the absence of CD11a and CD18. NOD mutant mice are resistant to diabetes to 34 weeks of age. Histological evaluation of the pancreas, at 18 weeks of age, reveals no insulitis.
This strain may be useful for in vivo analysis of leukocyte integrin-dependent adhesion in inflammatory disease models, specifically Type 1 Diabetes.
A targeting construct was made with a 10kb segment of the Itgb2 gene containing exon 2 and 3 and a neomycin-resistance gene (neo) casette with a short version of the RNA polymerase II promoter and the bovine growth hormone polyadenylation signal. The neomycin casette disrupts the splice-acceptor site of exon 3. The Itgb2 mutation maintained on C57BL/6 background was transferred through 8 backcross generations onto the NOD/ShiLtJ background. In 2011, the T1DR received this strain at generation N8F20.
|Allele Name||targeted mutation 2, Baylor College of Medicine|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||beta2-; CD18-; CD18 null; Itgb2-|
|Gene Symbol and Name||Itgb2, integrin beta 2|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|Molecular Note||A neomycin cassette disrupted the 5' boundary of exon 3. No protein was synthesized from this allele.|
|Mutations Made By|| |
Dr. Arthur Beaudet, Baylor College of Medicine
Recommended mating scheme - Heterozygous female x homozygous male. The homozygous female will usually produce 1 litter prior to becoming non productive.
When using the NOD.Cd18 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #016897 in your Materials and Methods section.