These Cdc14bdeltaE2 mutant mice are deficient in Cdc14b and exhibit premature aging and impaired DNA damage repair.
Dr. Pumin Zhang, Baylor College of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cdc14b | CDC14 cell division cycle 14B |
Cdc14b encodes a nuclear phosphatase that has been implicated in nuclear organization, centriole duplication, mitotic exit, cell cycle regulation, and DNA damage repair. Mice that are homozygous for this targeted mutation allele are viable, fertile, and normal in size.
Truncated gene product (mRNA), that is not expected to produce functional protein, is detected by RT-PCR analysis of MEFs from homozygotes. Homozygotes develop cataracts and kyphosis much earlier than wildtype controls and exhibit impaired contextual fear conditioning. Homozygous females exhibit impaired fertility, with smaller litter sizes than wildtype controls. MEFs isolated from homozygotes display defective DNA damage repair and slowed growth rate in later-passages.
A FRT site flanked targeting vector containing Kan/Neo selection cassette with an upstream loxP site was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted downstream of exon 2. This construct was electroporated into 129P2/OlaHsd-derived E14Tg2a.4embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to Meox2-Cre mice to excise exon 2. The donating investigator reported that mice were backcrossed to C57BL/6 mice for 7 generations (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Allele Name | targeted mutation 1.2, Pumin Zhang |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cdc14bdeltaE2 |
Gene Symbol and Name | Cdc14b, CDC14 cell division cycle 14B |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 13 |
Molecular Note | Cre-mediated recombination removed exon 2 and the neo cassette. RT-PCR confirmed the expression of two truncated transcripts that are not predicted to produce a functional protein. |
Mutations Made By | Dr. Pumin Zhang, Baylor College of Medicine |
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygous mice have reduced fertility (particularly for homozygous females) and smaller litter sizes. If maintaining the colony by breeding homozygous mice together, a larger colony size is recommended to account for this phenotype.
When using the B6.129P2(Cg)-Cdc14btm1.2Pzg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016896 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Cdc14b<tm1.2Pzg> |
Frozen Mouse Embryo | B6.129P2(Cg)-Cdc14b<tm1.2Pzg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2(Cg)-Cdc14b<tm1.2Pzg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2(Cg)-Cdc14b<tm1.2Pzg>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2(Cg)-Cdc14b<tm1.2Pzg>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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