The leg dragger mutation provides a viable mutation in kinesin family member 1A that causes pre-wean hindleg paralysis, whereas a targeted null mutation causes perinatal lethality. This strain provides a model for autosomal recessive spastic paraplegia 30.
Read More +Genetic Background | Generation |
---|---|
F7+pN1F3
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous (Not Specified) | Kif1a | kinesin family member 1A |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
The H2 haplotype of the live Kif1algdg colony (2017) has not been assessed.
Leg dragger homozygotes are slightly smaller than their unaffected littermates and lose most of the use of their rear legs such that they drag their rear legs and pull themselves along with their front legs to move. This phenotype can be detected as early as 2 weeks of age and is evident by 3 weeks of age. A few homozygotes nearing wean age are found to roll over and over in a struggle to right themselves. When raised by their tails they do not splay their legs outward but rather cross the front pair and the rear pair. Auditory brainstem response analysis of one homozygous animal at 18 days of age showed severe hearing loss and no others were tested. Heterozygotes appear normal and fertile but produce slightly fewer homozygotes than the 25% expected when intercrossed.
The leg dragger mutation (Kif1algdg) arose spontaneously on the C3.NB-H2p H2-T18c?/SnJ background. Before 2015, sperm was cryopreserved from heterozygous males at generation +F7.
To establish a live Kif1algdg mutant colony in 2017, The Jackson Laboratory Repository used an aliquot of the frozen sperm to fertilize oocytes from C3H/HeJ inbred females (Stock No. 000659 [H2k]). Rederived Kif1algdg mice were then bred to wildtype littermates or to C3H/HeJ mice. The H2 haplotype of the live Kif1algdg colony has not been assessed.
Allele Name | leg dragger |
---|---|
Allele Type | Spontaneous (Not Specified) |
Allele Synonym(s) | |
Gene Symbol and Name | Kif1a, kinesin family member 1A |
Gene Synonym(s) | |
Strain of Origin | C3.NB-H2p H2-T18c?/SnJ |
Chromosome | 1 |
Molecular Note | A spontaneous C-to-T (G-to-A on forward strand) point mutation at chromosome 1 position 93,076,218 bp (GRCm38/mm10) causes a p.L181F mutation (Ctc to Ttc). |
When using the legdragger mouse strain in a publication, please cite the originating article(s) and include JAX stock #016894 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Kif1a<lgdg> |
Frozen Mouse Embryo | C3.Cg-Kif1a<lgdg>/GrsrJ | $2595.00 |
Frozen Mouse Embryo | C3.Cg-Kif1a<lgdg>/GrsrJ | $2595.00 |
Frozen Mouse Embryo | C3.Cg-Kif1a<lgdg>/GrsrJ | $3373.50 |
Frozen Mouse Embryo | C3.Cg-Kif1a<lgdg>/GrsrJ | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.