The leg dragger mutation provides a viable mutation in kinesin family member 1A that causes pre-wean hindleg paralysis, whereas a targeted null mutation causes perinatal lethality. This strain provides a model for autosomal recessive spastic paraplegia 30.Read More +
The H2 haplotype of the live Kif1algdg colony (2017) has not been assessed.
Leg dragger homozygotes are slightly smaller than their unaffected littermates and lose most of the use of their rear legs such that they drag their rear legs and pull themselves along with their front legs to move. This phenotype can be detected as early as 2 weeks of age and is evident by 3 weeks of age. A few homozygotes nearing wean age are found to roll over and over in a struggle to right themselves. When raised by their tails they do not splay their legs outward but rather cross the front pair and the rear pair. Auditory brainstem response analysis of one homozygous animal at 18 days of age showed severe hearing loss and no others were tested. Heterozygotes appear normal and fertile but produce slightly fewer homozygotes than the 25% expected when intercrossed.
The leg dragger mutation (Kif1algdg) arose spontaneously on the C3.NB-H2p H2-T18c?/SnJ background. Before 2015, sperm was cryopreserved from heterozygous males at generation +F7.
To establish a live Kif1algdg mutant colony in 2017, The Jackson Laboratory Repository used an aliquot of the frozen sperm to fertilize oocytes from C3H/HeJ inbred females (Stock No. 000659 [H2k]). Rederived Kif1algdg mice were then bred to wildtype littermates or to C3H/HeJ mice. The H2 haplotype of the live Kif1algdg colony has not been assessed.
|Allele Name||leg dragger|
|Allele Type||Spontaneous (Not Specified)|
|Gene Symbol and Name||Kif1a, kinesin family member 1A|
|Strain of Origin||C3.NB-H2p H2-T18c?/SnJ|
|Molecular Note||A spontaneous C-to-T (G-to-A on forward strand) point mutation at chromosome 1 position 93,076,218 bp (GRCm38/mm10) causes a p.L181F mutation (Ctc to Ttc).|
When using the legdragger mouse strain in a publication, please cite the originating article(s) and include JAX stock #016894 in your Materials and Methods section.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.