Overview

Also Known As: legdragger

The leg dragger mutation provides a viable mutation in kinesin family member 1A that causes pre-wean hindleg paralysis, whereas a targeted null mutation causes perinatal lethality. This strain provides a model for autosomal recessive spastic paraplegia 30.

Genetic overview

Genetic Background	Generation
	F7+pN1F3 (2019-03-12 00:00:00)

Kif1a^lgdg

Allele Type	Gene Symbol	Gene Name
Spontaneous (Not Specified)	Kif1a	kinesin family member 1A

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Research Applications

Developmental Biology Research
Neurobiology Research

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Base Price

Starting at:

$278.00 Domestic price for female 4-week

356.51 Domestic price for breeder pair

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Details

Important Note

The H2 haplotype of the live Kif1a^lgdg colony (2017) has not been assessed.

Detailed Description

Leg dragger homozygotes are slightly smaller than their unaffected littermates and lose most of the use of their rear legs such that they drag their rear legs and pull themselves along with their front legs to move. This phenotype can be detected as early as 2 weeks of age and is evident by 3 weeks of age. A few homozygotes nearing wean age are found to roll over and over in a struggle to right themselves. When raised by their tails they do not splay their legs outward but rather cross the front pair and the rear pair. Auditory brainstem response analysis of one homozygous animal at 18 days of age showed severe hearing loss and no others were tested. Heterozygotes appear normal and fertile but produce slightly fewer homozygotes than the 25% expected when intercrossed.

Development

The leg dragger mutation (Kif1a^lgdg) arose spontaneously on the C3.NB-H2^p H2-T18^c?/SnJ background. Before 2015, sperm was cryopreserved from heterozygous males at generation +F7.

To establish a live Kif1a^lgdg mutant colony in 2017, The Jackson Laboratory Repository used an aliquot of the frozen sperm to fertilize oocytes from C3H/HeJ inbred females (Stock No. 000659 [H2^k]). Rederived Kif1a^lgdg mice were then bred to wildtype littermates or to C3H/HeJ mice. The H2 haplotype of the live Kif1a^lgdg colony has not been assessed.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Harris BS; Fairfield HE; Reinholdt LG; Berry ML; Bergstrom DE; Schroeder DG; Cox GA. 2016. Two viable spontaneous mutation in Kif1a MGI Direct Data SubmissionMGI: J:229662

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Genetics

Kif1a^lgdg

Allele Symbol: Kif1a^lgdg

Allele Name	leg dragger
Allele Type	Spontaneous (Not Specified)
Allele Synonym(s)	Kif1a^lgdg; leg dragger
Gene Symbol and Name	Kif1a, kinesin family member 1A
Gene Synonym(s)	C2orf20; ATSV; kinesin family member 1 (C. elegans unc104 homolog); N-3 kinesin; C630002N23Rik; UNC104; MRD9; Gm1626; Kns1; HSN2C; Kns1; LOC381283; gene model 1626, (NCBI); SPG30; C630002N23Rik; RIKEN cDNA C630002N23 gene
Strain of Origin	C3.NB-H2^p H2-T18^c?/SnJ
Chromosome	1
Molecular Note	A spontaneous C to T point transition at Chromosome 1 position 93,076,218 bp (GRCm38/mm10) causes this L181F mutation (Ctc to Ttc).

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

hereditary spastic paraplegia 30

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Kif1a^lgdg related

Developmental Biology Research
- Embryonic Lethality (Homozygous)
  - incomplete
Neurobiology Research
- Ataxia (Movement) Defects
- Hearing Defects
- Neurodegeneration

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Kif1a^lgdg/Kif1a^lgdg
C3.Cg-Kif1a/GrsrJ

behavior/neurological phenotype

abnormal limb posture
- wWhen raised by their tails they do not splay their legs outward and some cross the front pair and the rear pair

abnormal locomotor coordination
- a few homozygotes nearing wean age are found to roll over and over in a struggle to right themselves

hindlimb paralysis
- progressive paralysis can first present as early as 2 weeks of age and is eveident by 3 weeks of age, homozygotes lose most of the use of their rear legs such that they drag their rear legs and pull themselves along with their front legs to move

growth/size/body region phenotype

decreased body size
- homozygotes are slightly smaller than littermate controls

hearing/vestibular/ear phenotype

increased or absent threshold for auditory brainstem response
- one homozygote assessed at 18 days of age was found to have severe hearing loss

mortality/aging

prenatal lethality, incomplete penetrance
- of 743 pups born from heterozygous intercrosses 139 were mutant, 6 were born dead, 2 were found dead after birth, and 12 were missing before wean age, which is a yield of approximately 21% assuming all dead and missing were mutant, less than the 25% expected

References

Harris BS; Fairfield HE; Reinholdt LG; Berry ML; Bergstrom DE; Schroeder DG; Cox GA. 2016. Two viable spontaneous mutation in Kif1a MGI Direct Data SubmissionMGI: J:229662

Additional - Kif1a^lgdg related

Technical Support

Contact Technical Support

Genotyping Protocols
- Sanger sequencing: Kif1a^lgdg-SEQ
- End Point Analysis: Kif1a^lgdgL181F-EP
- Genotyping resources and troubleshooting
Dietary Information
- New Diet as of March 2015: Lab Diet® 5K0Q (6% fat)
Mating System
- Heterozygote x Heterozygote
Citation
When using the legdragger mouse strain in a publication, please cite the originating article(s) and include JAX stock #016894 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Kif1a<lgdg>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: legdragger

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Kif1algdg

Allele Symbol: Kif1algdg

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Kif1algdg related

Mammalian Phenotype Terms by Genotype

Genotype: Kif1algdg/Kif1algdgC3.Cg-Kif1a/GrsrJ

behavior/neurological phenotype

growth/size/body region phenotype

hearing/vestibular/ear phenotype

mortality/aging

References

Additional - Kif1algdg related

Genotyping Protocols

Dietary Information

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Kif1a^lgdg

Allele Symbol: Kif1a^lgdg

Genotype: Kif1a^lgdg related

Genotype: Kif1a^lgdg/Kif1a^lgdg
C3.Cg-Kif1a/GrsrJ

Additional - Kif1a^lgdg related