B6N.Cg-Adgra2^tm1.1Bstc/J

Stock number: 016881
Research areas: Cardiovascular Research, Research Tools

Description

These Gpr124^flox mutant mice possess loxP sites flanking exon 1 of the G protein-coupled receptor 124 (Gpr124) targeted gene. This strain may be useful for studying neurovasculature development and cerebrovascular disease.

Detailed description

These Gpr124^flox mutant mice possess loxP sites flanking exon 1 of the G protein-coupled receptor 124 (Gpr124) targeted gene. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. GPR124 is overexpressed in tumor vasculature and is required for blood vessel migration, formation of the blood brain barrier, and expansion of the cerebral cortex. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue. This strain may be useful for studying neurovasculature development and cerebrovascular disease.

When bred to mice carrying Tg(Tek-cre)12Flv (Stock No. 004128), Cre recombinase expression in the endothelial cells results in abnormal angiogenesis.

Development

A targeting vector was designed to insert a loxP site upstream of exon 1, and a frt-loxP-neomycin selection cassette (neo)-frt-loxP cassette downstream of exon 1 of the G protein-coupled receptor 124 (Gpr124) targeted gene. The construct was electroporated into (C57BL/6J X 129S4/SvJae)F1-derived V6.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred to mice carrying the β-actin flp deleter allele, on a C57BL/6 background, to remove the frt-flanked neo cassette. This line was backcrossed to C57BL/6NCr for at least 12 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.

Allele

Symbol: Adgra2^tm1.1Bstc
Name: targeted mutation 1.1, Brad St Croix
MGI accession ID: MGI:4949901
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Adgra2
Marker name: adhesion G protein-coupled receptor A2
Chromosome: 8
Strain of origin: (C57BL/6J x 129S4/SvJae)F1
Molecular note: A loxP site was inserted upstream of exon 1 and an FRT-loxP-neo-FRT-loxP cassette was introduced downstream of exon 1 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 1 floxed.