These Gpr124flox mutant mice possess loxP sites flanking exon 1 of the G protein-coupled receptor 124 (Gpr124) targeted gene. This strain may be useful for studying neurovasculature development and cerebrovascular disease.
Brad St. Croix, NATIONAL INSTITUTES OF HEALTH
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Adgra2 | adhesion G protein-coupled receptor A2 |
These Gpr124flox mutant mice possess loxP sites flanking exon 1 of the G protein-coupled receptor 124 (Gpr124) targeted gene. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. GPR124 is overexpressed in tumor vasculature and is required for blood vessel migration, formation of the blood brain barrier, and expansion of the cerebral cortex. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue. This strain may be useful for studying neurovasculature development and cerebrovascular disease.
When bred to mice carrying Tg(Tek-cre)12Flv (Stock No. 004128), Cre recombinase expression in the endothelial cells results in abnormal angiogenesis.
A targeting vector was designed to insert a loxP site upstream of exon 1, and a frt-loxP-neomycin selection cassette (neo)-frt-loxP cassette downstream of exon 1 of the G protein-coupled receptor 124 (Gpr124) targeted gene. The construct was electroporated into (C57BL/6J X 129S4/SvJae)F1-derived V6.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred to mice carrying the β-actin flp deleter allele, on a C57BL/6 background, to remove the frt-flanked neo cassette. This line was backcrossed to C57BL/6NCr for at least 12 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.
Allele Name | targeted mutation 1.1, Brad St Croix |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Gpr124flox |
Gene Symbol and Name | Adgra2, adhesion G protein-coupled receptor A2 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6J x 129S4/SvJae)F1 |
Chromosome | 8 |
Molecular Note | A loxP site was inserted upstream of exon 1 and an FRT-loxP-neo-FRT-loxP cassette was introduced downstream of exon 1 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 1 floxed. |
Mutations Made By | Brad St. Croix, NATIONAL INSTITUTES OF HEALTH |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6N.Cg-Adgra2tm1.1Bstc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016881 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gpr124<tm1.1Bstc> |
Frozen Mouse Embryo | B6N.Cg-Adgra2<tm1.1Bstc>/J | $2595.00 |
Frozen Mouse Embryo | B6N.Cg-Adgra2<tm1.1Bstc>/J | $2595.00 |
Frozen Mouse Embryo | B6N.Cg-Adgra2<tm1.1Bstc>/J | $3373.50 |
Frozen Mouse Embryo | B6N.Cg-Adgra2<tm1.1Bstc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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