These floxed mutant mice have loxP sites flanking exon 4 of the bone morphogenetic protein 4 gene (Bmp4). This strain may be useful for generating mutations in applications related to bone morphogenetic protein signaling pathways, bone biology, cardiovascular biology and cancer.
James F Martin, Baylor College of Medicine
Mice homozygous for this Bmp4floxneo allele have loxP sites flanking exon 4 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous mature peptide deleted in the cre-expressing tissues resulting in a null allele. Mice homozygous for the Bmp4floxneo allele are viable and fertile. These mutant mice may be useful in generating conditional mutations for studying the role of Bmp4 in bone morphogenetic protein signaling pathways, bone biology, cardiovascular biology, and cancer (Bmp4 misregulation is associated with cancer cell motility/invasiveness and epithelial-to-mesenchymal transition/transformation [EMT]).
For example, when crossed to a strain expressing Cre recombinase in early limb bud mesenchyme and in a subset of craniofacial mesenchyme (see Stock No. 005584), this mutant mouse strain may be useful in studies of limb patterning and skeletogenesis.
A targeting vector was designed to insert a loxP site upstream of exon 4, and a second loxP site followed by an frt-flanked PGK-neo cassette just downstream of exon 4 of the targeted gene. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6 females to establish the colony. Mice heterozygous for the Bmp4floxneo allele were bred together to generate homozygotes prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, James F Martin|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Bmp4clc; Bmp4floxneo|
|Gene Symbol and Name||Bmp4, bone morphogenetic protein 4|
|Strain of Origin||129S4/SvJaeSor|
|Molecular Note||A loxP site was introduced upstream of exon 4 and another loxP site followed by a FRT-flanked neo was inserted downstream of exon 4. PCR was used to confirm recombination.|
|Mutations Made By|| |
James Martin, Baylor College of Medicine
When maintaining a live colony, homozygous mice may be bred together.
When using the Bmp4floxneo mouse strain in a publication, please cite the originating article(s) and include JAX stock #016878 in your Materials and Methods section.