B6;129S4-Bmp4^tm1Jfm/J

Stock number: 016878
Product name: Bmp4^floxneo
Strain synonyms: Bmp4^clc
Research areas: Cancer Research, Cardiovascular Research, Cell Biology Research, Developmental Biology Research, Internal/Organ Research, Research Tools

Description

These floxed mutant mice have loxP sites flanking exon 4 of the bone morphogenetic protein 4 gene (Bmp4). This strain may be useful for generating mutations in applications related to bone morphogenetic protein signaling pathways, bone biology, cardiovascular biology and cancer.

Detailed description

Mice homozygous for this Bmp4^floxneo allele have loxP sites flanking exon 4 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous mature peptide deleted in the cre-expressing tissues resulting in a null allele. Mice homozygous for the Bmp4^floxneo allele are viable and fertile. These mutant mice may be useful in generating conditional mutations for studying the role of Bmp4 in bone morphogenetic protein signaling pathways, bone biology, cardiovascular biology, and cancer (Bmp4 misregulation is associated with cancer cell motility/invasiveness and epithelial-to-mesenchymal transition/transformation [EMT]).

For example, when crossed to a strain expressing Cre recombinase in early limb bud mesenchyme and in a subset of craniofacial mesenchyme (see Stock No. 005584), this mutant mouse strain may be useful in studies of limb patterning and skeletogenesis.

Development

A targeting vector was designed to insert a loxP site upstream of exon 4, and a second loxP site followed by an frt-flanked PGK-neo cassette just downstream of exon 4 of the targeted gene. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6 females to establish the colony. Mice heterozygous for the Bmp4^floxneo allele were bred together to generate homozygotes prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Bmp4^tm1Jfm
Name: targeted mutation 1, James F Martin
MGI accession ID: MGI:3041440
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Bmp4
Marker name: bone morphogenetic protein 4
Chromosome: 14
Strain of origin: 129S4/SvJaeSor
Molecular note: A loxP site was introduced upstream of exon 4 and another loxP site followed by a FRT-flanked neo was inserted downstream of exon 4. PCR was used to confirm recombination.