Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. While no gene product (mRNA) is detected by in situ hybridization and RT-PCR analysis of taste buds of the circumvallate papillae with a probe specific for the transmembrane motif 9 to the C terminus, an incomplete gene product (mRNA) is detected by in situ hybridization analysis with a probe specific for transmembrane motif 2 to the C terminus. Apical localization of PKD2L1 protein is absent in the mutant taste buds. Recorded chorda tympani and glossopharyngeal nerve responses of homozygotes to taste stimuli is similar to wildtype controls. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background. No fluorescence from the GFP in the knock in construct has been observed in the mice.
When crossed to mice carrying a Pkd2l1tm1Hmat knockout mutation (See Stock No. 016853), the double homozygous mutant mice exhibit reduced chorda tympani nerve responses to sour and acid taste stimuli.
A targeting vector containing IRES-EGFP, and a self-excising floxed (ACN) neo cassette was used to disrupt exons 26 through 31, which encode the transmembrane motif 7 to 11. The construct was electroporated into 129S/SvEv and C57BL/6 derived EF1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 5 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1.1, Keiko Abe|
|Allele Type||Targeted (Reporter, Null/Knockout)|
|Gene Symbol and Name||Pkd1l3, polycystic kidney disease 1 like 3|
|Strain of Origin||129S/SvEv x C57BL/6|
|Molecular Note||Exons 26 through 31 with an IRES, an EGFP, and a self-excising floxed neo cassette. These exons encode transmembrane domains 7 through 11. In situ hybridization confirmed the absence of transcript expression in the taste buds of the circumvallate papillae.|
|Mutations Made By|| |
Hiroaki Matsunami, Duke University Medical Center
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.Cg-Pkd1l3tm1.1Abek/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016850 in your Materials and Methods section.