STOCK Mir9-1^tm1Mtm/Mmjax

Stock number: 016846
Research areas: Cell Biology Research, Neurobiology Research, Research Tools

Description

These microRNA 9-1 conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. LacZ expression is observed in embryonic brain and spinal cord. This mutant mouse strain may be useful in studies of microRNA biology.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR9-1 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR9-1 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in embryonic brain and spinal cord. This mutant mouse strain may be useful in studies of microRNA biology.

Development

A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the miR9-1 stem loop, with one loxP site placed immediately downstream of the miR9-1 stem loop. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeric mice were crossed with a mouse of mixed genetic background (which incorporated FVB), then crossed to inbred C57BL/6 for two generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Allele

Symbol: Mir9-1^tm1Mtm
Name: targeted mutation 1, Michael McManus
MGI accession ID: MGI:5314759
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter; Null/Knockout
Marker symbol: Mir9-1
Marker name: microRNA 9-1
Chromosome: 3
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: When combined with a Cre-recombinase-expressing strain, LacZ expression is observed in embryonic brain and spinal cord.
Molecular note: A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop.