Overview

These microRNA 9-1 conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. LacZ expression is observed in embryonic brain and spinal cord. This mutant mouse strain may be useful in studies of microRNA biology.

Donating Investigator

Michael T McManus, University of California, San Francisco

Genetic overview

Genetic Background	Generation

Mir9-1^tm1Mtm

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), Reporter, Null/Knockout)	Mir9-1	microRNA 9-1

View Genetics

Research Applications

Neurobiology Research
Research Tools
Cell Biology Research

View All Research Applications

Details

Detailed Description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain,
the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR9-1 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR9-1 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in embryonic brain and spinal cord. This mutant mouse strain may be useful in studies of microRNA biology.

Development

A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the miR9-1 stem loop, with one loxP site placed immediately downstream of the miR9-1 stem loop. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeric mice were crossed with a mouse of mixed genetic background (which incorporated FVB), then crossed to inbred C57BL/6 for two generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	When combined with a Cre-recombinase-expressing strain, LacZ expression is observed in embryonic brain and spinal cord.

Control Suggestions

Approximate Controls

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Genetics

Mir9-1^tm1Mtm

Allele Symbol: Mir9-1^tm1Mtm

Allele Name	targeted mutation 1, Michael McManus
Allele Type	Targeted (Conditional ready (e.g. floxed), Reporter, Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Mir9-1, microRNA 9-1
Gene Synonym(s)
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	When combined with a Cre-recombinase-expressing strain, LacZ expression is observed in embryonic brain and spinal cord.
Strain of Origin	129P2/OlaHsd
Chromosome	3
Molecular Note	A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - loxP-flanked Sequences
  - loxP-flanked Sequences: Test/Reporter
- lacZ expression in neural tissue
Research Tools
- Cre-lox System
  - loxP-flanked Sequences
  - loxP-flanked Sequences: Test/Reporter
- FLP-FRT System
  - FRT-flanked Sequences
- lacZ Expression
Cell Biology Research
- microRNA
  - loxP-flanked microRNA sequence

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Mir9-1^tm1Mtm/Mir9-1^tm1Mtm
involves: 129P2/OlaHsd * C57BL/6

mammalian phenotype

no phenotypic analysis

References

Additional - Mir9-1^tm1Mtm related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Mir9-1
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice have been bred as heterozygotes, however, the Donating Investigator indicates that homozygotes are viable and fertile.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Mir9-1^tm1Mtm/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34801 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Mir9-1tm1Mtm

Allele Symbol: Mir9-1tm1Mtm

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Mir9-1tm1Mtm/Mir9-1tm1Mtminvolves: 129P2/OlaHsd * C57BL/6

mammalian phenotype

References

Additional - Mir9-1tm1Mtm related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Mir9-1^tm1Mtm

Allele Symbol: Mir9-1^tm1Mtm

Genotype: Mir9-1^tm1Mtm/Mir9-1^tm1Mtm
involves: 129P2/OlaHsd * C57BL/6

Additional - Mir9-1^tm1Mtm related