B6.FVB(129)-Tg(Alb1-cre)1Dlr/J

Stock number: 016832
Product name: Alb1-cre
Research areas: Developmental Biology Research, Research Tools

Description

The Alb1-cre transgene was designed with a Cre recombinase gene under control of Alb promoter/enhancer elements. These mice may be useful for studying the liver specific deletion of target genes.

Detailed description

The Alb1-cre transgene was designed with a Cre recombinase gene under control of albumin (Alb) promoter/enhancer elements. Hemizygous Alb1-cre mice are viable, fertile, and normal in size, with cre expression directed specifically to the liver. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing hepatic cells of the offspring. For example, when these mice are bred to Igf1^tm1Dlr-containing mice (Stock No. 012663 or Stock No. 016831), the removal of IGF1 expression in the liver causes a 75% reduction in serum IGF-1levels. These mice may be useful for studying the liver specific deletion of target genes.

Development

The Alb1-Cre transgene was designed with a Cre recombinase gene under control of albumin (Alb) promoter/enhancer elements. The construct was microinjected into fertilized FVB/N oocytes, and founder mice were bred to FVB/NJ mice. The resulting mice were then crossed to B6.129-Igf1^tm1Dlr mice. The resulting offspring were then backcrossed to an C57BL/6J for at least 9 generations. Upon arrival, the mice were bred to C57BL/6J inbred mice (Stock No. 000664) for one generation and floxed allele was selectively bred out of the line.

Allele

Symbol: Tg(Alb1-cre)1Dlr
Name: transgene insertion 1, Derek LeRoith
MGI accession ID: MGI:2176946
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Alb1-cre)1Dlr
Marker name: transgene insertion 1, Derek LeRoith
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: liver
Molecular note: This transgene expresses Cre recombinase in the liver, under the control of an albumin promoter (species not specified).