These floxed mutant mice possess loxP sites flanking exon 8 of the Gabrg2 gene. This strain may be useful for generating conditional mutations for studying the role of gamma2 subunit of the GABA-A receptor in neurophysiology and major depression.
Bernhard Luscher, Pennsylvania State University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Gabrg2 | gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 |
These mice possess loxP sites on either side of exon 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 8 deleted in the cre-expressing tissue(s).
When bred to a strain with Cre recombinase expression in glutamatergic neurons, this mutant mouse strain may be useful in studies of postsynaptic clustering of GABA-A receptors during synaptogenesis.
A loxP site flanked targeting vector containing a loxP site and FRT site flanked PGK-neo cassette. This selection cassette was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted upstream of exon 8. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. The resulting chimeric mice were crossed to FLP recombinase expressing transgenic mice (on a 129X1/SvJ x B6SJLF1 mixed background) to remove the selection cassette. Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reports that mice were then backcrossed to 129X1/SvJ (obtained from RCC in Fullinsdorf, Switzerland) for more than 40 generations (see SNP results below) prior to sending albino males to The Jackson Laboratory Repository in 2011. Upon arrival, males were bred with 129X1/SvJ females (Stock No. 000691) for one generation to establish the live colony. Mice homozygous for the mutation were then bred together to maintain the live colony.
A 27 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, was performed on the F3 generation living colony at The Jackson Laboratory Repository. This revealed 5 of 27 markers, one each on chromosomes 2, 7, 11, 15 and 17, that were not fixed for 129 allele-type (e.g., still segregating for C57BL/6;SJL allele-type markers). These data suggest the mice were backcrossed to 129X1/SvJ for fewer generations than reported prior to arrival at The Jackson Laboratory Repository, and the mice are most likely now a mix of 129 (~80%) and C57BL/6;SJL (~20%).
Allele Name | targeted mutation 2, Bernhard Luscher |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | fgamma2 |
Gene Symbol and Name | Gabrg2, gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 11 |
Molecular Note | Exon 8 was left flanked by single loxP sites after an frt flanked neo cassette was excised via Flp-mediated recombination. A single flp site was left immediately downstream of the 3' loxP site. |
Mutations Made By | Bernhard Luscher, Pennsylvania State University |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the STOCK Gabrg2tm2Lusc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016830 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gabrg2<tm2Lusc> |
Frozen Mouse Embryo | STOCK Gabrg2<tm2Lusc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gabrg2<tm2Lusc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gabrg2<tm2Lusc>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Gabrg2<tm2Lusc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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