129S.Cg-Tg(Hoxb7-rtTA*M2)2Cos/J

Stock number: 016567
Research areas: Developmental Biology Research, Internal/Organ Research, Research Tools

Description

RS-HTA2 transgenic mice have the homeobox B7 promoter/enhancer sequences driving expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein. These mice may be useful for the inducible expression of genes throughout the Wolffian duct, ureteric bud (UB) epithelium, and developing kidney.

Detailed description

RS-HTA2 transgenic mice have the homeobox B7 promoter/enhancer sequences driving expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein. Hemizygotes are viable, fertile, and normal in size. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. When bred to B6;SJL-Tg(tetop-lacZ)2Mam/J mice (Stock No. 002621), adult mice carrying both transgenes, which were maintained on Dox during pre and postnatal life, show strong expression of βgal in the renal collecting duct system, and embryos display strong expression throughout the Wolffian duct, ureteric bud, vas deferens, epididymis and seminal vesicles. These mice may be useful for the inducible expression of genes throughout the Wolffian duct, ureteric bud (UB) epithelium, and developing kidney.

Development

The Hoxb7-rtTA*M2 transgene was designed with the homeobox B7 (Hoxb7) promoter directing expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein, followed by a simian virus 40 (SV40) polyadenylation (polyA) signal. The transgene was microinjected into fertilized (B6CBA) F1 oocytes, and the resulting offspring were bred to 129S6/SvEvTac mice. These RS-HTA2 mice were backcrossed to 129S6/SvEvTac for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.

Allele

Symbol: Tg(Hoxb7-rtTA*M2)2Cos
Name: transgene insertion 2, Franklin Costantini
MGI accession ID: MGI:4950470
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Hoxb7-rtTA*M2)2Cos
Marker name: transgene insertion 2, Franklin Costantini
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Molecular note: The Hoxb7-rtTA*M2 transgene was designed with the homeobox B7 (Hoxb7) promoter directing expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein, followed by a simian virus 40 (SV40) polyadenylation (polyA) signal.