<i>RS-HTA2</i> transgenic mice have the homeobox B7 promoter/enhancer sequences driving expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein.  Hemizygotes are viable, fertile, and normal in size.  When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. When bred to B6;SJL-Tg(tetop-lacZ)2Mam/J mice (Stock No. <a href="https://www.jax.org/strain/002621">002621</a>), adult mice carrying both transgenes, which were maintained on Dox during pre and postnatal life, show strong expression of &beta;gal in the renal collecting duct system, and embryos display strong expression throughout the Wolffian duct, ureteric bud, vas deferens, epididymis and seminal vesicles.  These mice may be useful for the inducible expression of genes throughout the Wolffian duct, ureteric bud (UB) epithelium, and developing kidney.

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<i>RS-HTA2</i> transgenic mice have the homeobox B7 promoter/enhancer sequences driving expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein.  These mice may be useful for the inducible expression of genes throughout the Wolffian duct, ureteric bud (UB) epithelium, and developing kidney.

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