B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J

Stock number: 016532
Product name: CAGs-rtTA3
Research areas: Research Tools

Description

When combined with a tetO/TRE transgene, this CAG-rtTA mutation enables conditional expression in the presence of doxycycline. This CAGs-rtTA3 transgenic strain is a component of a novel system applying conditional RNA interference (RNAi) to knock out targeted genes.

Detailed description

The CAGs-rtTA3 transgene has a CAG (cytomegalovirus (CMV) early enhancer element and chicken beta-actin) promoter driving widespread expression of reverse tetracycline-controlled transactivator 3 (rtTA3). High rtTA3 expression is observed in most tissues examined. Mice hemizygous for the CAGs-rtTA3 transgene are viable and fertile.

When crossed with mice carrying a TRE/tetO promoter-directed gene of interest, progeny may be treated with doxycycline to initiate conditional expression.

When crossed with TRE-GFP-shRNAmir (short hairpin microRNA) transgenic mice, the donating laboratory was able to conditionally regulate RNA interference (RNAi) with doxycycline with high efficiency and selectively mark gene knockouts with GFP fluorescence.

Development

A DNA fragment incorporating a CAG (cytomegalovirus (CMV) early enhancer element and chicken beta-actin) promoter and highly sensitive reverse tetracycline-controlled transactivator 3 (rtTA3) was introduced to FVB embryos via pronuclear injection. This strain had genetic background contributions from FVB, C57BL/6 and 129. Transgenic mice were subsequently backcrossed to C57BL/6NCrl inbred mice for four generations by the donating laboratory prior to sending to The Jackson Laboratory Repository. Upon arrival, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the live colony. The transgene is integrated on mouse Chromosome 8.

Allele

Symbol: Tg(CAG-rtTA3)4288Slowe
Name: transgene insertion 4288, Scott Lowe
MGI accession ID: MGI:5000298
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(CAG-rtTA3)4288Slowe
Marker name: transgene insertion 4288, Scott Lowe
Chromosome: 8
Strain of origin: FVB
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: rtTA3 expression is observed in most tissues.
Molecular note: The transgene contains a DNA fragment incorporating a CAG (cytomegalovirus (CMV) early enhancer element and chicken beta-actin) promoter and highly sensitive reverse tetracycline-controlled transactivator 3 (rtTA3). Line 4288 was generated. The transgene inserted on chromosome 8.