When combined with a tetO/TRE transgene, this CAG-rtTA mutation enables conditional expression in the presence of doxycycline. This CAGs-rtTA3 transgenic strain is a component of a novel system applying conditional RNA interference (RNAi) to knock out targeted genes.
Scott W Lowe, Memorial Sloan Kettering Cancer Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
The CAGs-rtTA3 transgene has a CAG (cytomegalovirus (CMV) early enhancer element and chicken beta-actin) promoter driving widespread expression of reverse tetracycline-controlled transactivator 3 (rtTA3). High rtTA3 expression is observed in most tissues examined. Mice hemizygous for the CAGs-rtTA3 transgene are viable and fertile.
When crossed with mice carrying a TRE/tetO promoter-directed gene of interest, progeny may be treated with doxycycline to initiate conditional expression.
When crossed with TRE-GFP-shRNAmir (short hairpin microRNA) transgenic mice, the donating laboratory was able to conditionally regulate RNA interference (RNAi) with doxycycline with high efficiency and selectively mark gene knockouts with GFP fluorescence.
A DNA fragment incorporating a CAG (cytomegalovirus (CMV) early enhancer element and chicken beta-actin) promoter and highly sensitive reverse tetracycline-controlled transactivator 3 (rtTA3) was introduced to FVB embryos via pronuclear injection. This strain had genetic background contributions from FVB, C57BL/6 and 129. Transgenic mice were subsequently backcrossed to C57BL/6NCrl inbred mice for four generations by the donating laboratory prior to sending to The Jackson Laboratory Repository. Upon arrival, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the live colony. The transgene is integrated on mouse Chromosome 8.
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
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Site of Expression | rtTA3 expression is observed in most tissues. |
Allele Name | transgene insertion 4288, Scott Lowe |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | CAGs-rtTA3 |
Gene Symbol and Name | Tg(CAG-rtTA3)4288Slowe, transgene insertion 4288, Scott Lowe |
Gene Synonym(s) | |
Promoter | CMV, cytomegalovirus, human |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | rtTA3 expression is observed in most tissues. |
Strain of Origin | FVB |
Chromosome | 8 |
Molecular Note | The transgene contains a DNA fragment incorporating a CAG (cytomegalovirus (CMV) early enhancer element and chicken beta-actin) promoter and highly sensitive reverse tetracycline-controlled transactivator 3 (rtTA3). Line 4288 was generated. The transgene inserted on chromosome 8. |
When maintained as a live colony, hemizygous mice may be bred with wildtype (noncarrier) mice from the colony or with C57BL/6NJ inbred mice (Stock No. 005304).
When using the CAGs-rtTA3 mouse strain in a publication, please cite the originating article(s) and include JAX stock #016532 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(CAG-rtTA3)4288Slowe |
Frozen Mouse Embryo | B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J | $2595.00 |
Frozen Mouse Embryo | B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J | $2595.00 |
Frozen Mouse Embryo | B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J | $3373.50 |
Frozen Mouse Embryo | B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J | $3373.50 |
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