The FOXO1/GFP allele contains a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA) upstream of a forkhead box O1 (Foxo1)-green fluorescent protein (GFP) fusion protein. These mice may be useful for generating conditional mutations for studying the role of PI3K-AKT signaling pathway on insulin resistance, glucose intolerance, diabetes, and aging.
Joel K Elmquist, UT Southwestern Med Center at Dallas
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
The FOXO1/GFP allele contains a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA) upstream of a forkhead box O1 (Foxo1)-green fluorescent protein (GFP) fusion protein. Homozygotes are viable and fertile. In the absence of Cre, reporter gene expression is prevented by the STOP sequence. When bred to mice that express Cre recombinase, offspring will have the floxed-STOP cassette deleted in the cre-expressing tissue(s), resulting in FOXO1/GFP expression in Cre-recombinase-expressing cells. FOXO1 is a component of the phosphoinositide 3-kinase (PI3K)-AKT pathway, and shuttles between the nucleus and the cytoplasm upon activation/inhibition by AKT. The PI3K-AKT signaling pathway is activated after administration of insulin and leptin. Exclusion of FoxO1 from the nucleus upon AKT phosphorylation allows visualization of activated/inactivated AKT by monitoring sub-cellular localization of the FOXO1-GFP fusion protein. These mice may be useful for generating conditional mutations for studying the role of PI3K-AKT signaling pathway on insulin resistance, glucose intolerance, diabetes, and aging.
The FOXO1/GFP targeting vector was designed with a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA) upstream of a forkhead box O1 (Foxo1)-green fluorescent protein (GFP) fusion protein. This vector was inserted into the Gt(ROSA)26Sor locus and was electroporated into 129-derived embryonic stem (ES) cells. The resulting chimeric mice were bred to C57BL/6J mice. Upon arrival at The Jackson Laboratory, mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | Foxo1, forkhead box O1, mouse, laboratory |
Site of Expression | When bred to mice that express Cre recombinase, the FOXO1-GFP fusion protein will be expressed in cells where promoters driving Cre recombinase are expressed. |
Allele Name | targeted mutation 1, Joel K Elmquist |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | Rosa26-FoxoGFP |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Foxo1, forkhead box O1, mouse, laboratory |
Site of Expression | When bred to mice that express Cre recombinase, the FOXO1-GFP fusion protein will be expressed in cells where promoters driving Cre recombinase are expressed. |
Strain of Origin | 129 |
Chromosome | 6 |
Molecular Note | A floxed neo-STOP cassette followed by a Foxo1/GFP fusion was inserted into the locus. Expression of the reporter construct can only be achieved by cre-mediated removal of the neo-STOP cassette. |
Mutations Made By | Joel Elmquist, UT Southwestern Med Center at Dallas |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129-Gt(ROSA)26Sortm1(Foxo1/GFP)Jke/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016262 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm1(Foxo1/GFP)Jke> |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(Foxo1/GFP)Jke>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(Foxo1/GFP)Jke>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(Foxo1/GFP)Jke>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(Foxo1/GFP)Jke>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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