Hoxb7-myr-Venus transgenic mice express a myristoylated yellow fluorescent protein, myr-Venus under the direction of a homeobox B7 promoter/enhancer. These mice may be useful for visualizing individual cells in the membranes of the Wolffian duct and branching ureteric bud of the kidney.
Frank Costantini, Columbia University Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Hoxb7-myr-Venus transgenic mice have the homeobox B7 promoter/enhancer sequences driving expression of a myristoylated yellow fluorescent protein, myr-Venus. Hemizygotes are viable, fertile, and normal in size. Under control of Hoxb7, myr-Venus labels the membranes of individual cells in the Wolffian duct and branching ureteric bud (UB) of the kidney during all stages of urogenital development. This strain allows for the visualization of the shapes and arrangements of individual cells. In contrast, mice containing the Tg(Hoxb7-EGFP)33Cos allele (Stock No. 016251) are useful for viewing tissues specific to the UB lineage.
The Hoxb7-myr-Venus transgene was designed with a myristoylated yellow fluorescent protein, myr-Venus, driven by homeobox B7 promoter/enhancer sequences, followed by a human β-globin sequence, and a polyadenylation site. The transgene was microinjected into fertilized (B6xCBA)F2 oocytes and mice from founder line 17 were bred to (B6xCBA)F1 mice to establish a colony. The donating investigator reported that these mice were then backcrossed to 129/SvEv mice for at least 4 years (see SNP note below). Upon arrival at The Jackson Laboratory, transgenic mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 5 of the 27 markers throughout the genome were found to be segregating for C57BL/6 and CBA. These data suggest the mice sent to The Jackson Laboratory Repository were not completely backcrossed.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Site of Expression | Myr-Venus labels the membranes of individual cells in the Wolffian duct and branching ureteric bud (UB) of the kidney during all stages of urogenital development. |
Allele Name | transgene insertion 17, Franklin Costantini |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Hoxb7/myr-Venus |
Gene Symbol and Name | Tg(Hoxb7-Venus*)17Cos, transgene insertion 17, Franklin Costantini |
Gene Synonym(s) | |
Promoter | Hoxb7, homeobox B7, mouse, laboratory |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | Myr-Venus labels the membranes of individual cells in the Wolffian duct and branching ureteric bud (UB) of the kidney during all stages of urogenital development. |
Strain of Origin | (C57BL/6J x CBA/J)F2 |
Chromosome | UN |
Molecular Note | The promoter was used to drive kidney-specific expression of a myristoylated Venus reporter. Lines 10 and 17 were created and line 17 was used for further research. |
Mutations Made By | Frank Costantini, Columbia University Medical Center |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to 129S1/SvImJ inbred mice (Stock No. 002448).
When using the STOCK Tg(Hoxb7-Venus*)17Cos/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016252 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Hoxb7-Venus*)17Cos |
Frozen Mouse Embryo | STOCK Tg(Hoxb7-Venus*)17Cos/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Hoxb7-Venus*)17Cos/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Hoxb7-Venus*)17Cos/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Hoxb7-Venus*)17Cos/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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