STOCK Tg(Hoxb7-Venus*)17Cos/J

Stock number: 016252
Research areas: Research Tools

Description

Hoxb7-myr-Venus transgenic mice express a myristoylated yellow fluorescent protein, myr-Venus under the direction of a homeobox B7 promoter/enhancer. These mice may be useful for visualizing individual cells in the membranes of the Wolffian duct and branching ureteric bud of the kidney.

Detailed description

Hoxb7-myr-Venus transgenic mice have the homeobox B7 promoter/enhancer sequences driving expression of a myristoylated yellow fluorescent protein, myr-Venus. Hemizygotes are viable, fertile, and normal in size. Under control of Hoxb7, myr-Venus labels the membranes of individual cells in the Wolffian duct and branching ureteric bud (UB) of the kidney during all stages of urogenital development. This strain allows for the visualization of the shapes and arrangements of individual cells. In contrast, mice containing the Tg(Hoxb7-EGFP)33Cos allele (Stock No. 016251) are useful for viewing tissues specific to the UB lineage.

Development

The Hoxb7-myr-Venus transgene was designed with a myristoylated yellow fluorescent protein, myr-Venus, driven by homeobox B7 promoter/enhancer sequences, followed by a human β-globin sequence, and a polyadenylation site. The transgene was microinjected into fertilized (B6xCBA)F2 oocytes and mice from founder line 17 were bred to (B6xCBA)F1 mice to establish a colony. The donating investigator reported that these mice were then backcrossed to 129/SvEv mice for at least 4 years (see SNP note below). Upon arrival at The Jackson Laboratory, transgenic mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 5 of the 27 markers throughout the genome were found to be segregating for C57BL/6 and CBA. These data suggest the mice sent to The Jackson Laboratory Repository were not completely backcrossed.

Allele

Symbol: Tg(Hoxb7-Venus*)17Cos
Name: transgene insertion 17, Franklin Costantini
MGI accession ID: MGI:4415296
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Hoxb7-Venus*)17Cos
Marker name: transgene insertion 17, Franklin Costantini
Chromosome: UN
Strain of origin: (C57BL/6J x CBA/J)F2
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish
Site of expression: Myr-Venus labels the membranes of individual cells in the Wolffian duct and branching ureteric bud (UB) of the kidney during all stages of urogenital development.
Molecular note: The promoter was used to drive kidney-specific expression of a myristoylated Venus reporter. Lines 10 and 17 were created and line 17 was used for further research.