TopCreERT2 transgenic mice express a CreERT2 fusion protein under the control of three copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) response element and a c-fos minimal promoter. This strain may useful for studying the Wnt signaling pathway.
Douglas Epstein, University of Pennsylvania
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
TopCreERT2 transgenic mice express a CreERT2 fusion protein under the control of three copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) response element and a c-fos minimal promoter. Mice hemizygous for the transgene are viable, fertile, and normal in size. Wnt (wingless-related MMTV) family members are required for triggering embryonic axis formation and for proper development. Wnt signaling results in phosphorylation and nuclear localization of β-catenin, a transcriptional co-activator protein, which, together with the TCF/Lef family of transcription factors, induces the transcription of downstream genes. TopCreERT2 transgenic mice contain three copies of nuclear localized TCF/Lef1 DNA binding sites, which provides increased sensitivity to Wnt/β-catenin signaling. Cre-ERT2 fusion gene activity is inducible; observed at high levels following tamoxifen administration. When TopCreERT2 transgenic mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in cells of the double mutant offspring with active Wnt-signaling. Specifically, the TopCreERT2 transgene directs cre expression in the dorsal regions of the otic vesicle of the inner ear. This strain may useful for studying the Wnt signaling pathway.
The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.
The TopCreERT2 transgene was designed with three copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) DNA binding site and a minimal c-fos promoter driving expression of a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). The transgene was microinjected into fertilized (B6J x SJL)F1 oocytes, and founder mice were bred to outbred CD1 mice to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | This transgene directs cre expression in the dorsal regions of the otic vesicle of the inner ear. |
Allele Name | transgene insertion 1, Douglas J Epstein |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Tg(Fos-cre/ERT2)1Dje; TopCreERT2 |
Gene Symbol and Name | Tg(TCF/Lef1-cre/ERT2)1Dje, transgene insertion 1, Douglas J Epstein |
Gene Synonym(s) | |
Promoter | Lef1, lymphoid enhancer binding factor 1, mouse, laboratory |
Promoter | Fos, FBJ osteosarcoma oncogene, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | This transgene directs cre expression in the dorsal regions of the otic vesicle of the inner ear. |
Strain of Origin | Not Specified |
Chromosome | UN |
Molecular Note | Three consensus LEF/TCF-binding motifs upstream of a minimal Fos promoter were used to drive expression of a tamoxifen inducible cre construct. |
Mutations Made By | Douglas Epstein, University of Pennsylvania |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony.
When using the STOCK Tg(TCF/Lef1-cre/ERT2)1Dje/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016236 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(TCF/Lef1-cre/ERT2)1Dje |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-cre/ERT2)1Dje/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-cre/ERT2)1Dje/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-cre/ERT2)1Dje/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-cre/ERT2)1Dje/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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