The coding region of the Nanog homeobox (<i>Nanog</i>) is replaced by a GFP-IRES-puro-cassette in these mice, abolishing gene expression.  NANOG is normally expressed in embryonic stem cells (ESCs) and functions as a key factor in maintaining pluripotency.  Heterozygous mutant mice are viable and fertile, while homozygotes are embryonic lethal. Mouse embryonic fibroblasts (MEFs) derived from these GFP reporter mice are useful for identifying induced pluripotent stem (iPS) cell formation. 

A GFP-IRES-puro-cassette replaces the coding region of the <i>Nanog</i> gene in these mice. Mouse embryonic fibroblasts containing this mutation may be useful for identifying induced pluripotent stem cells.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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