B6.Cg-Tg(Drd1a-tdTomato)6Calak/J

Stock number: 016204
Product name: Drd1a-tdTomato line 6
Research areas: Neurobiology Research, Research Tools

Description

These transgenic BAC Drd1a-tdTomato mice contain a mouse Drd1a promoter directing the expression of a modified DsRed fluorescent protein, tdTomato. These mice may be useful for specific visualization of striatonigral projection neurons and for studying dopamine-sensitive behaviors.

Detailed description

These mutant mice harbor a transgenic BAC containing the mouse dopamine receptor D1A (Drd1a) promoter directing expression of a modified DsRed fluorescent protein, tdTomato. These Drd1a-tdTomato mice (founder line 6) express tdTomato in striatonigral neurons in a more accurate and specific manner than seen in previous founder lines. Hemizygous mice are viable, fertile and normal in size. These mice may be useful for specific visualization of striatonigral projection neurons and for studying dopamine-sensitive behaviors.

Development

A BAC (#RP23-47M2) containing the entire mouse dopamine receptor D1A (Drd1a) gene was customized to contain a modified 5' DsRed fluorescent protein (tdTomato). The BAC was microinjected into fertilized B6SJL F1 oocytes. Drd1a-tdTomato mice from founder line 6 were bred to C57BL/6 inbred mice for at least 6 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Drd1a-tdTomato)6Calak
Name: transgene insertion 6, Nicole Calakos
MGI accession ID: MGI:4947687
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Drd1a-tdTomato)6Calak
Marker name: transgene insertion 6, Nicole Calakos
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: RFP,Red Fluorescent Protein,coral
Site of expression: Drd1a-tdTomato mice express tdTomato in striatonigral neurons.
Molecular note: A BAC (#RP23-47M2) containing the entire mouse dopamine receptor D1A (Drd1a) gene was customized to contain a modified 5' DsRed fluorescent protein (tdTomato).