This compound transgenic strain was created by co-injecting a BAC clone encoding the entire mouse Per1 locus and a transgenic vector in which the Per1 promoter drives expression of luciferase (Per1:luc). Unlike most Per1:luc transgenic mice, expression of Per1:luc oscillates in the suprachiasmatic nuclei (SCN) as well as peripheral tissues in ex vivo cultures. This strain may be useful in studies of circadian rhythm.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
This compound transgenic strain was created by co-injecting a BAC clone encoding the entire mouse Per1 locus and a transgenic vector in which the Per1 promoter drives expression of luciferase (Per1:luc). The expression of Per1:luc oscillates in the suprachiasmatic nuclei (SCN) as well as peripheral tissues in ex vivo cultures (unlike most other Per1:luc transgenic strains which have weak rhythms in peripheral tissues).
An ~150 kb Research Genetics BAC clone encoding the entire mouse Per1 locus and a transgenic vector in which the Per1 promoter drives expression of luciferase were co-injected into BTBR embryos. Resultant mice were backcrossed to C57BL/6J for 6 generations by the donating laboratory.
Expressed Gene | luc, luciferase, firefly |
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Expressed Gene | Per1, period circadian clock 1, mouse, laboratory |
Site of Expression | Luciferase is expressed in a pattern recapitulating that of circadian rhythms in the suprachiasmatic nuclei (SCN). |
Allele Name | transgene insertion 1, Joseph S Takahashi |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Per1-luc,Per1)1Jt, transgene insertion 1, Joseph S Takahashi |
Gene Synonym(s) | |
Promoter | Per1, period circadian clock 1, mouse, laboratory |
Expressed Gene | luc, luciferase, firefly |
Expressed Gene | Per1, period circadian clock 1, mouse, laboratory |
Site of Expression | Luciferase is expressed in a pattern recapitulating that of circadian rhythms in the suprachiasmatic nuclei (SCN). |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | An ~150 kb Research Genetics BAC clone encoding the entire mouse Per1 locus was co-injected with a transgenic vector in which the Per1 promoter drives expression of luciferase. Line 1 was generated. |
When maintained in a live colony, hemizygotes may be bred.
When using the B6.BTBR-Tg(Per1-luc,Per1)1Jt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016187 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Per1-luc,Per1)1Jt |
Frozen Mouse Embryo | B6.BTBR-Tg(Per1-luc Per1)1Jt/J | $2595.00 |
Frozen Mouse Embryo | B6.BTBR-Tg(Per1-luc Per1)1Jt/J | $2595.00 |
Frozen Mouse Embryo | B6.BTBR-Tg(Per1-luc Per1)1Jt/J | $3373.50 |
Frozen Mouse Embryo | B6.BTBR-Tg(Per1-luc Per1)1Jt/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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