JAX Mouse Strain Datasheet - 016184

B6.129(Cg)-Csnk1e^tm1.1Asil/J

Stock No:: 016184

Cryo Recovery

Overview

These knockout Csnk1e (casein kinase 1, epsilon) mice exhibit a small but significant period lengthening compared to their wildtype counterparts. This strain may be useful in studies of circadian rhythm.

Donating Investigator

Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr

Genetic overview

Genetic Background	Generation

Csnk1e^tm1.1Asil

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Csnk1e	casein kinase 1, epsilon

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Research Applications

Neurobiology Research

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Deletion of exon 4 of the Csnk1e (casein kinase 1, epsilon) gene creates a frameshift that results in a premature stop codon. RT-PCR and western blotting confirm the gene knockout. Homozygotes exhibit a small but significant period lengthening compared to their wildtype counterparts (24.0+/- 0.06 versus 23.6 +/- 0.05 hours, respectively). Homozygotes are viable and fertile with no overt deleterious phenotype.

Development

A targeting vector was used to introduce the tau mutation (nucleotide C12555T; amino acid C178T) to exon 4, a loxP site to intron 3, and a NeoTK (neomycin/thymidine kinase) selection cassette flanked by loxP sites to intron 4. R1 embyronic stem (ES) cells derived from (129X1/SvJ x 129S1/Sv)F1- Kitl⁺ mice were used to create mutant animals. The floxed selection cassette was deleted from homologously recombined ES cell clones by transient transfection with a cre-expressing plasmid, leaving exon 4 flanked by loxP sites. Resultant heterozygous tau mice were crossed with cre-deleter animals of unknown genetic background to excise exon 4. This strain was backcrossed to C57BL/6J for more than 8 generations by the donating laboratory.

Selected References

Meng QJ; Logunova L; Maywood ES; Gallego M; Lebiecki J; Brown TM; Sladek M; Semikhodskii AS; Glossop NR; Piggins HD; Chesham JE; Bechtold DA; Yoo SH; Takahashi JS; Virshup DM; Boot-Handford RP; Hastings MH; Loudon AS. 2008. Setting clock speed in mammals: the CK1 epsilon tau mutation in mice accelerates circadian pacemakers by selectively destabilizing PERIOD proteins. Neuron 58(1):78-88. PubMed: 18400165 MGI: J:135186

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Genetics

Csnk1e^tm1.1Asil

Allele Symbol: Csnk1e^tm1.1Asil

Allele Name	targeted mutation 1.1, Andrew S I Loudon
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	CK1epsilon^-
Gene Symbol and Name	Csnk1e, casein kinase 1, epsilon
Gene Synonym(s)	AI426939; AI551861; AW457082; CK1epsilon; CKI epsilon; CKIepsilon; HCKIE; KC1epsilon; expressed sequence AI426939; expressed sequence AI551861; expressed sequence AW457082; tau
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Chromosome	15
Molecular Note	Germ line, cre-mediated recombination was used to remove exon 4 causing a frame shift and premature stop codon. The absence of protein product was confirmed by western blot analysis on brain extracts.
Mutations Made By	Dr. Andrew Loudon, University of Manchester

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Circadian Rhythms

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Csnk1e^tm1.1Asil/Csnk1e⁺
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

behavior/neurological phenotype

behavior/neurological phenotype
- exhibited a wild-type like circadian period
- mutant mice are viable and fertile with no overt deleterious phenotype

Genotype: Csnk1e^tm1.1Asil/Csnk1e^tm1.1Asil
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

behavior/neurological phenotype

prolonged circadian period
- a small but significant circadian period lengthening compared to their wild-type counterpart
- mutant mice are viable and fertile with no overt deleterious phenotype

References

Meng QJ; Logunova L; Maywood ES; Gallego M; Lebiecki J; Brown TM; Sladek M; Semikhodskii AS; Glossop NR; Piggins HD; Chesham JE; Bechtold DA; Yoo SH; Takahashi JS; Virshup DM; Boot-Handford RP; Hastings MH; Loudon AS. 2008. Setting clock speed in mammals: the CK1 epsilon tau mutation in mice accelerates circadian pacemakers by selectively destabilizing PERIOD proteins. Neuron 58(1):78-88. PubMed: 18400165 MGI: J:135186

Additional - Csnk1e^tm1.1Asil related

Meng QJ; Maywood ES; Bechtold DA; Lu WQ; Li J; Gibbs JE; Dupre SM; Chesham JE; Rajamohan F; Knafels J; Sneed B; Zawadzke LE; Ohren JF; Walton KM; Wager TT; Hastings MH; Loudon AS. 2010. Entrainment of disrupted circadian behavior through inhibition of casein kinase 1 (CK1) enzymes. Proc Natl Acad Sci U S A 107(34):15240-5. PubMed: 20696890 MGI: J:163807
Zhou L; Bryant CD; Loudon A; Palmer AA; Vitaterna MH; Turek FW. 2014. The circadian clock gene Csnk1e regulates rapid eye movement sleep amount, and nonrapid eye movement sleep architecture in mice. Sleep 37(4):785-93, 793A-793C. PubMed: 24744456 MGI: J:237463

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Csnk1e<tm1.1Asil>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Selected References

Csnk1etm1.1Asil

Allele Symbol: Csnk1etm1.1Asil

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Csnk1etm1.1Asil/Csnk1e+involves: 129S1/Sv * 129X1/SvJ * C57BL/6

behavior/neurological phenotype

Genotype: Csnk1etm1.1Asil/Csnk1etm1.1Asilinvolves: 129S1/Sv * 129X1/SvJ * C57BL/6

behavior/neurological phenotype

References

Additional - Csnk1etm1.1Asil related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Csnk1e^tm1.1Asil

Allele Symbol: Csnk1e^tm1.1Asil

Genotype: Csnk1e^tm1.1Asil/Csnk1e⁺
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Genotype: Csnk1e^tm1.1Asil/Csnk1e^tm1.1Asil
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Additional - Csnk1e^tm1.1Asil related