These knockout Csnk1e (casein kinase 1, epsilon) mice exhibit a small but significant period lengthening compared to their wildtype counterparts. This strain may be useful in studies of circadian rhythm.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Csnk1e | casein kinase 1, epsilon |
Deletion of exon 4 of the Csnk1e (casein kinase 1, epsilon) gene creates a frameshift that results in a premature stop codon. RT-PCR and western blotting confirm the gene knockout. Homozygotes exhibit a small but significant period lengthening compared to their wildtype counterparts (24.0+/- 0.06 versus 23.6 +/- 0.05 hours, respectively). Homozygotes are viable and fertile with no overt deleterious phenotype.
A targeting vector was used to introduce the tau mutation (nucleotide C12555T; amino acid C178T) to exon 4, a loxP site to intron 3, and a NeoTK (neomycin/thymidine kinase) selection cassette flanked by loxP sites to intron 4. R1 embyronic stem (ES) cells derived from (129X1/SvJ x 129S1/Sv)F1- Kitl+ mice were used to create mutant animals. The floxed selection cassette was deleted from homologously recombined ES cell clones by transient transfection with a cre-expressing plasmid, leaving exon 4 flanked by loxP sites. Resultant heterozygous tau mice were crossed with cre-deleter animals of unknown genetic background to excise exon 4. This strain was backcrossed to C57BL/6J for more than 8 generations by the donating laboratory.
Allele Name | targeted mutation 1.1, Andrew S I Loudon |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CK1epsilon- |
Gene Symbol and Name | Csnk1e, casein kinase 1, epsilon |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 15 |
Molecular Note | Germ line, cre-mediated recombination was used to remove exon 4 causing a frame shift and premature stop codon. The absence of protein product was confirmed by western blot analysis on brain extracts. |
Mutations Made By | Dr. Andrew Loudon, University of Manchester |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129(Cg)-Csnk1etm1.1Asil/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016184 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Csnk1e<tm1.1Asil> |
Frozen Mouse Embryo | B6.129(Cg)-Csnk1e<tm1.1Asil>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Csnk1e<tm1.1Asil>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Csnk1e<tm1.1Asil>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129(Cg)-Csnk1e<tm1.1Asil>/J Frozen Embryo | $3373.50 |
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