B6.129(Cg)-Csnk1e^tm1.1Asil/J

Stock number: 016184
Research areas: Neurobiology Research

Description

These knockout Csnk1e (casein kinase 1, epsilon) mice exhibit a small but significant period lengthening compared to their wildtype counterparts. This strain may be useful in studies of circadian rhythm.

Detailed description

Deletion of exon 4 of the Csnk1e (casein kinase 1, epsilon) gene creates a frameshift that results in a premature stop codon. RT-PCR and western blotting confirm the gene knockout. Homozygotes exhibit a small but significant period lengthening compared to their wildtype counterparts (24.0+/- 0.06 versus 23.6 +/- 0.05 hours, respectively). Homozygotes are viable and fertile with no overt deleterious phenotype.

Development

A targeting vector was used to introduce the tau mutation (nucleotide C12555T; amino acid C178T) to exon 4, a loxP site to intron 3, and a NeoTK (neomycin/thymidine kinase) selection cassette flanked by loxP sites to intron 4. R1 embyronic stem (ES) cells derived from (129X1/SvJ x 129S1/Sv)F1- Kitl⁺ mice were used to create mutant animals. The floxed selection cassette was deleted from homologously recombined ES cell clones by transient transfection with a cre-expressing plasmid, leaving exon 4 flanked by loxP sites. Resultant heterozygous tau mice were crossed with cre-deleter animals of unknown genetic background to excise exon 4. This strain was backcrossed to C57BL/6J for more than 8 generations by the donating laboratory.

Allele

Symbol: Csnk1e^tm1.1Asil
Name: targeted mutation 1.1, Andrew S I Loudon
MGI accession ID: MGI:3793321
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Csnk1e
Marker name: casein kinase 1, epsilon
Chromosome: 15
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Germ line, cre-mediated recombination was used to remove exon 4 causing a frame shift and premature stop codon. The absence of protein product was confirmed by western blot analysis on brain extracts.