These transgenic mice express the wildtype allele of the mouse Nr1d1 (nuclear receptor subfamily 1, group D, member 1) gene under the control of a tetracycline-responsive promoter element (TRE; tetO).
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
These transgenic mice express hemagglutinin-tagged Nr1d1 (nuclear receptor subfamily 1, group D, member 1) cDNA under the control of a tetracycline-responsive element (TRE; tetO)/minimal cytomegalovirus (CMV) promoter. When hemizygotes are bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled tranactivator protein (tTA) under the regulation of tissue-specific promoters, transgene expression can be conditionally regulated with doxycycline.
A transgenic vector carrying full-length Nr1d1 cDNA (first 134 bp from mouse preceded by two hemagglutinin (HA) tags and followed by the remaining rat sequence) downstream of seven tetracycline-responsive elements (TRE;tetO) and a minimal CMV promoter was introduced to fertilized C57BL/6J mouse oocytes. These mice have been maintained on a C57BL/6J background by the donating lab.
Expressed Gene | Nr1d1, nuclear receptor subfamily 1, group D, member 1, rat |
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Expressed Gene | Nr1d1, nuclear receptor subfamily 1, group D, member 1, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion 1, Ueli Schibler |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | tetORevErba; Tg(tetO/CMV-Nr1d1)1Schb |
Gene Symbol and Name | Tg(tetO-Nr1d1)1Schb, transgene insertion 1, Ueli Schibler |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Nr1d1, nuclear receptor subfamily 1, group D, member 1, rat |
Expressed Gene | Nr1d1, nuclear receptor subfamily 1, group D, member 1, mouse, laboratory |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | The transgene contains the first 134 bp from mouse full-length cDNA preceded by two hemagglutinin (HA) tags and followed by the remaining rat sequence, all downstream of seven tetracycline-responsive elements (TRE;tetO) and a minimal CMV promoter. Line 1 was generated. |
When maintained as a live colony, homozygotes or hemizygotes may be bred.
When using the C57BL/6-Tg(tetO-Nr1d1)1Schb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016181 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(tetO-Nr1d1)1Schb |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-Nr1d1)1Schb/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-Nr1d1)1Schb/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-Nr1d1)1Schb/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-Nr1d1)1Schb/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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