C57BL/6-Tg(tetO-Nr1d1)1Schb/J

Stock number: 016181
Research areas: Neurobiology Research, Research Tools

Description

These transgenic mice express the wildtype allele of the mouse Nr1d1 (nuclear receptor subfamily 1, group D, member 1) gene under the control of a tetracycline-responsive promoter element (TRE; tetO).

Detailed description

These transgenic mice express hemagglutinin-tagged Nr1d1 (nuclear receptor subfamily 1, group D, member 1) cDNA under the control of a tetracycline-responsive element (TRE; tetO)/minimal cytomegalovirus (CMV) promoter. When hemizygotes are bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled tranactivator protein (tTA) under the regulation of tissue-specific promoters, transgene expression can be conditionally regulated with doxycycline.

Development

A transgenic vector carrying full-length Nr1d1 cDNA (first 134 bp from mouse preceded by two hemagglutinin (HA) tags and followed by the remaining rat sequence) downstream of seven tetracycline-responsive elements (TRE;tetO) and a minimal CMV promoter was introduced to fertilized C57BL/6J mouse oocytes. These mice have been maintained on a C57BL/6J background by the donating lab.

Allele

Symbol: Tg(tetO-Nr1d1)1Schb
Name: transgene insertion 1, Ueli Schibler
MGI accession ID: MGI:4950924
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-Nr1d1)1Schb
Marker name: transgene insertion 1, Ueli Schibler
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: Nr1d1,nuclear receptor subfamily 1, group D, member 1,mouse, laboratory; Nr1d1,nuclear receptor subfamily 1, group D, member 1,rat
Molecular note: The transgene contains the first 134 bp from mouse full-length cDNA preceded by two hemagglutinin (HA) tags and followed by the remaining rat sequence, all downstream of seven tetracycline-responsive elements (TRE;tetO) and a minimal CMV promoter. Line 1 was generated.