Full-length mouse Cry2 (cryptochrome 2 (photolyase-like)) cDNA is under the transcriptional control of a tetracycline-responsive promoter in these transgenic mice. This strain may be useful in studies of circadian rhythm.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
Full-length mouse Cry2 (cryptochrome 2 (photolyase-like)) cDNA is the under transcriptional control of a tetracycline-responsive promoter in these transgenic mice. When crossed with animals expressing the tetracycline-controlled transactivator (tTA) or reverse tetracycline-controlled transactivator (rtTA) protein under the control of tissue-specific promoters, transgene expression can be regulated in the offspring by the administration of doxycycline.
When crossed with an Scg2 (secretogranin II)-promoter driven tTA strain (see Stock No. 008284), CRY2 is expressed in the suprachiasmatic nucleus and brain, causing a circadian pattern with a shortened period. This effect can be inhibited by low doses of doxycycline in the drinking water. The effects of low, but not high, doses of doxycycline are completely reversible and lead to a rapid reactivation of the transgene.
A transgenic vector carrying the full-length wildtype mouse Cry2 cDNA downstream of a tetracycline-responsive promoter (TRE;tetO) was introduced to fertilized C57BL/6J mouse oocytes. Originally maintained on a C57BL/6J-CD1 mixed genetic background, these mice have been backcrossed to C57BL/6J for more than 6 generations by the donating lab.
Expressed Gene | Cry2, cryptochrome 2 (photolyase-like), mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 3, Joseph S Takahashi |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | tetO-Cry2 |
Gene Symbol and Name | Tg(tetO-Cry2)3Jt, transgene insertion 3, Joseph S Takahashi |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Cry2, cryptochrome 2 (photolyase-like), mouse, laboratory |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | The transgene contains the full-length wildtype mouse Cry2 cDNA downstream of a tetracycline-responsive promoter (TRE;tetO). Line 3 was generated. |
When maintained as a live colony, hemizygotes may be bred.
When using the B6(Cg)-Tg(tetO-Cry2)3Jt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016178 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(tetO-Cry2)3Jt |
Frozen Mouse Embryo | B6(Cg)-Tg(tetO-Cry2)3Jt/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Tg(tetO-Cry2)3Jt/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Tg(tetO-Cry2)3Jt/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Tg(tetO-Cry2)3Jt/J Frozen Embryo | $3373.50 |
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