Gfi1:Gfi1b knock-in mice express mouse Gfi1b (growth factor independent 1B) from the endogenous Gfi1 (growth factor independent 1) locus and may be useful in studies of deafness, hematopoiesis, and lymphopoiesis.
Tarik Moroy, IRCM
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted | Gfi1 | growth factor independent 1 transcription repressor |
These mutant mice express mouse Gfi1b (growth factor independent 1B) from the endogenous Gfi1 locus. Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. Higher expression levels of Gfi1b transcript is detected in inner ears as measured by RT-PCR analysis. The Gfi1b knock-in did not completely rescue the Gfi1 knock out phenotype (see STOCK No. 016161). Homozygotes have slightly fewer granulocytes in bone marrow, a small accumulation in bone marrow of immature myeloid cells and monocytes, and fewer mature circulating granulocytes when compared to wildtype controls. At 3 to 3.5 months in age, homozygotes are deaf, do not display a Preyer reflex, abnormal auditory brainstem response (over 100 dB at 8 kHz and over 90 dB at 16 and 32 kHz), and exhibit head bobbing and abnormal reaching response. In neonatal mutants, the cochlear inner hair cells are morphologically immature and the outer hair cells are smaller in size and disorganized with progressive degeneration. The cochlear phenotype is less severe in these mice when compared to the knock-out mice. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.
A targeting vector containing a loxP-flanked NEO selection cassette and Gfi1b (growth factor independent 1B) cDNA was used to disrupt exons 2 through 5, the entire coding region. The Gfi1b cDNA was inserted immediately downstream of the Gfi1 translation initiation codon. Correctly targeted ES cells were injected into recipient blastocysts. Heterozygote mice were crossed to CMV-cre transgenic mice (on the C57BL/6 background) to remove the floxed NEO selection cassette. The mice were then backcrossed to C57BL/6 for 14 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | Gfi1b, growth factor independent 1B, mouse, laboratory |
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Site of Expression |
Allele Name | targeted mutation 3, Tarik Moroy |
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Allele Type | Targeted |
Allele Synonym(s) | Gfi1:Gfi1b |
Gene Symbol and Name | Gfi1, growth factor independent 1 transcription repressor |
Gene Synonym(s) | |
Expressed Gene | Gfi1b, growth factor independent 1B, mouse, laboratory |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 5 |
Molecular Note | The coding region was replaced with growth factor independent 1B cDNA. |
Mutations Made By | Cyrus Khandanpour, IRCM |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Gfi1:Gfi1b knock-in mouse strain in a publication, please cite the originating article(s) and include JAX stock #016163 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gfi1<tm3(Gfib1)Tmo> |
Frozen Mouse Embryo | B6.129-Gfi1<tm3(Gfib1)Tmo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Gfi1<tm3(Gfib1)Tmo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Gfi1<tm3(Gfib1)Tmo>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Gfi1<tm3(Gfib1)Tmo>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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