These Zic2A986T mice possess an ENU-induced A to T transversion at nucleotide 986 of the zinc finger protein of the cerebellum 2 (Zic2) gene. These mutant mice may be useful in studying embryonic development.
Lee Niswander, University of Colorado, Denver
Mice heterozygous for the ENU-induced mutation, Zic2A986T, are viable and fertile, although homozygotes die before birth. These mice possess an A to T transversion at nucleotide 986 of the zinc finger protein of the cerebellum 2 (Zic2) gene. These mice exhibit spina bifida and 20% exhibit exencephaly (hind-mid-forebrain). These mutant mice may be useful in studying embryonic development.
Following multidose N-ethyl-N-nitrosourea (ENU) treatments to induce mutations in founder C57BL/6J mice, a forward genetic screen was utilized to identify mice exhibiting defects in the closure of embryonic fissures (including the neural tube, body wall, and face). Using a candidate gene approach a mutation was identified on Chromosome 14. Sequencing identified a single nucleotide polymorphism (SNP), resulting in an A to T transversion at nucleotide 986 (A986T) in the zinc finger protein of the cerebellum 2 (Zic2) gene. These Zic2A986T mice were bred to 129S1/SvImJ mice for at least 10 generations to maintain a colony. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
|Allele Name||mutation 1, Lee Niswander|
|Allele Type||Chemically induced (ENU) (Hypomorph)|
|Gene Symbol and Name||Zic2, zinc finger protein of the cerebellum 2|
|Strain of Origin||C57BL/6J|
|Molecular Note||ENU mutagenesis induced A to T transversion at position 986 (A986T) that changes glutamic acid to valine in the zinc finger domain. The protein level is not affected in this hypomorphic allele.|
When maintaining a live colony, heterozygous mice may be bred to wildtype from the colony or to 129S1/SvImJ mice (Stock No. 002448). Homozygous mice die before birth.
When using the 129S1.B6-Zic2m1Nisw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016158 in your Materials and Methods section.