This chromosome 4 congenic stock contains the C57BL/10SnJ derived interferon gene cluster, IFN, (Idd9.4) introgressed into the NOD/MrkTac background and exhibits significantly reduced diabetes incidence. This strain may be useful for positional mapping of the Idd9.4 loci and studying interferon candidate genes affecting autoimmunity.
Linda Wicker, University of Cambridge, UK
This NOD/MrkTac congenic strain, commonly referred to as NOD.B10-Idd9.4, line 9142 contains a 2Mb genomic segment from the diabetes resistant strain C57BL/10SnJ (Stock No. 000666) introgressed into chromosome 4 and is defined within markers D4Dil31 (87Mb) and D4Dil43 (88.5Mb). The C57BL/10SnJ genomic segment includes an interferon gene cluster thought to be involved with autoimmunity. The donating investigator indicates that homozygous congenic females exhibit significantly diminished diabetes development compared to NOD/MrkTac controls.
This strain may be useful for positional mapping of the Idd9.4 loci and studying interferon candidate genes affecting autoimmunity.
This strain, commonly called NOD.B10 Idd9.4 was produced by backcrossing NOD.c3c4 [Stock No. 010971 - NOD.B-(D3Mit93-D3Mit124)(D4Mit114-D4Mit142)/1112MrkJ], to NOD/MrkTac an additional 4 times until a recombination event isolating a congenic segment containing the type 1 IFN region occurred. The congenic interval is approximately 1.25Mb, with markers D4Dil31 and D4Dil43 being of C57BL/10SnJ origin, while markers D4Dil30 and D4Mit27 are of NOD origin. The C57BL/10SnJ segment contains the type 1 interferon gene cluster located on chromosome 4. A 1449 SNP chip shows this strain to be free of non-NOD segments outside of the congenic region. In 2011, the Type 1 Diabetes resource received this strain at N10F?.
|Gene Symbol and Name||Idd9, insulin dependent diabetes susceptibility 9|
|Strain of Origin||C57BL/10SnJ|
|Molecular Note||This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.|