STOCK Tg(Scgb1a1-rtTA)2Jaw/J

Stock number: 016145
Research areas: Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Mouse/Human Gene Homologs, Research Tools

Description

These CCSP-rtTA transgenic mice express the rtTA protein under the control of the rat Scgb1a1 gene promoter and provides an improved "Tet-On" tool that allows the inducible expression of genes in the developing and adult lung and respiratory epithelium.

Detailed description

These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), gene promoter. In situ hybridization detects rtTA mRNA in bronchial and type II epithelial cells of lung tissue from adult transgenic mice treated with doxycycline for seven days. Induction of transgene expression is detected as early as postconception day 14 when the pregnant female is treated with doxycycline. When mated to a second transgenic strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene can be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice hemizygous for the CCSP-rtTA transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.

This founder line has less non-doxycycline-dependent expression of rtTA than Founder Line 1 (See Stock No. 006232, and Stock No. 006242)

Development

A transgenic construct containing 3.7kb sequence of the rat Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), gene promoter, the reverse tetracycline regulatable transactivator protein (rtTA) gene, and 2.0kb sequence of the human grouth hormone gene, containing introns and polyadenylation site, was injected into mixed C57BL/6 and 129 background embryos. Mice from founder line 2 were bred to FVB/N mice. Upon arrival at The Jackson Laboratory, these mice were bred with C57BL/6J mice (Stock No. 000664) to establish the colony.

Allele

Symbol: Tg(Scgb1a1-rtTA)2Jaw
Name: transgene insertion 2, Jeffrey A Whitsett
MGI accession ID: MGI:5301895
Generation method: Transgenic
Attributes: Transactivator
Synonyms: SP-C-rtTA (Line 2)
Marker symbol: Tg(Scgb1a1-rtTA)2Jaw
Marker name: transgene insertion 2, Jeffrey A Whitsett
Chromosome: UN
Strain of origin: C57BL/6 and 129
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: Transgene is expressed in bronchial and type II epithelial cells of lung tissue from adult transgenic mice. Induction of transgene expression is detected as early as postconception day 14.
Molecular note: The transgenic construct contains 3.7kb sequence of the rat Scgb1a1 (secretoglobin, family 1A, member 1 (uteroglobin) gene promoter, the reverse tetracycline regulatable transactivator protein (rtTA) gene, and 2.0kb sequence of the human grouth hormone gene, containing introns and polyadenylation site. Line 2 was generated.