The mouse Defa2 (defensin, alpha, 2; also known as cryptdin 2 or CR2) promoter directs expression of FLAG-tagged Myd88 (myeloid differentiation primary response gene 88) to intestinal Paneth cells. This strain may be useful in studies of microbial/immune system interactions and antimicrobial transcriptional responses at the intestinal epithelial barrier.
Lora V. Hooper, UT Southwestern Medical Center, HHMI
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
The mouse Defa2 (defensin, alpha, 2; also known as cryptdin 2 or CR2) promoter directs expression of FLAG-tagged Myd88 (myeloid differentiation primary response gene 88) to the Paneth cells of the small intestine in this transgenic strain. Hemizygotes are viable and fertile and do not display any gross physical or behavioral abnormalities. Anti-FLAG antibodies may be used for immunohistochemical analysis. This strain may be useful in studies of microbial/immune system interactions and antimicrobial transcriptional responses at the intestinal epithelial barrier.
FLAG-tagged Myd88 was placed under the transcriptional control of nucleotides -6500 to +134 of the mouse Defa2 (defensin, alpha, 2; also known as cryptdin 2) gene. Human growth hormone (hGH, +3 to +2150) was placed immediately downstream to enhance transgenic expression, but is not transcribed. The transgenic vector was introduced to C57BL/6 embryos and resultant animals were maintained on a C57BL/6 genetic background by the donating laboratory.
Expressed Gene | Myd88, myeloid differentiation primary response gene 88, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1, Lora Hooper |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Cr2-FlagMyd88 |
Gene Symbol and Name | Tg(Defa2-Myd88)1Lvh, transgene insertion 1, Lora Hooper |
Gene Synonym(s) | |
Promoter | Defa2, defensin, alpha, 2, mouse, laboratory |
Expressed Gene | Myd88, myeloid differentiation primary response gene 88, mouse, laboratory |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | FLAG-tagged Myd88 was placed under the transcriptional control of nucleotides -6500 to +134 of the mouse Defa2 (defensin, alpha, 2; also known as cryptdin 2) gene. Human growth hormone (hGH, +3 to +2150) was placed immediately downstream to enhance transgenic expression, but is not transcribed. |
Mutations Made By | Lora Hooper, UT Southwestern Medical Center, HHMI |
When maintained as a live colony, hemizygotes may be bred with wildtype mice.
When using the C57BL/6-Tg(Defa2-Myd88)1Lvh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016133 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Defa2-Myd88)1Lvh |
Frozen Mouse Embryo | C57BL/6-Tg(Defa2-Myd88)1Lvh/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Defa2-Myd88)1Lvh/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Defa2-Myd88)1Lvh/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Defa2-Myd88)1Lvh/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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