C57BL/6-Tg(Defa2-Myd88)1Lvh/J

Stock number: 016133
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

The mouse Defa2 (defensin, alpha, 2; also known as cryptdin 2 or CR2) promoter directs expression of FLAG-tagged Myd88 (myeloid differentiation primary response gene 88) to intestinal Paneth cells. This strain may be useful in studies of microbial/immune system interactions and antimicrobial transcriptional responses at the intestinal epithelial barrier.

Detailed description

The mouse Defa2 (defensin, alpha, 2; also known as cryptdin 2 or CR2) promoter directs expression of FLAG-tagged Myd88 (myeloid differentiation primary response gene 88) to the Paneth cells of the small intestine in this transgenic strain. Hemizygotes are viable and fertile and do not display any gross physical or behavioral abnormalities. Anti-FLAG antibodies may be used for immunohistochemical analysis. This strain may be useful in studies of microbial/immune system interactions and antimicrobial transcriptional responses at the intestinal epithelial barrier.

Development

FLAG-tagged Myd88 was placed under the transcriptional control of nucleotides -6500 to +134 of the mouse Defa2 (defensin, alpha, 2; also known as cryptdin 2) gene. Human growth hormone (hGH, +3 to +2150) was placed immediately downstream to enhance transgenic expression, but is not transcribed. The transgenic vector was introduced to C57BL/6 embryos and resultant animals were maintained on a C57BL/6 genetic background by the donating laboratory.

Allele

Symbol: Tg(Defa2-Myd88)1Lvh
Name: transgene insertion 1, Lora Hooper
MGI accession ID: MGI:4941629
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Defa2-Myd88)1Lvh
Marker name: transgene insertion 1, Lora Hooper
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Myd88,myeloid differentiation primary response gene 88,mouse, laboratory
Molecular note: FLAG-tagged Myd88 was placed under the transcriptional control of nucleotides -6500 to +134 of the mouse Defa2 (defensin, alpha, 2; also known as cryptdin 2) gene. Human growth hormone (hGH, +3 to +2150) was placed immediately downstream to enhance transgenic expression, but is not transcribed.