These mice carry a targeted mutation of the Snca (synuclein, alpha) gene. Mice that are homozygous for the targeted mutation are viable and fertile. Expression of the targeted mutation allele has not been examined. Mutations in human SNCA that result in aberrant polymerization and fibrillar aggregations are associated with neurodegenerative synucleinopathies, such as Alzheimer's and Parkinson's diseases.
A targeting vector containing a floxed NEO cassette was used to disrupt exons 1 through 4. The construct was electroporated into C57BL/6NTac-derived B6-3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were crossed to C57BL/6NTac-Gt(ROSA)26Sortm16(cre)Arte mice to remove the floxed NEO cassette. The mice were maintained on the C57BL/6NTac background by the Donating Investigator. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.
|Allele Name||targeted mutation 1, The Michael J Fox Foundation|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Snca, synuclein, alpha|
|Strain of Origin||C57BL/6NTac|
|General Note||The was created in B6-3 embryonic stem (ES) cells.|
|Molecular Note||A targeting vector containing a floxed NEO cassette was used to disrupt exons 1 through 4. Correctly targeted chimeric animals were crossed to C57BL/6NTac-Gt(ROSA)26Sortm16(cre)Arte mice to remove the floxed NEO cassette.|
When maintaining a live colony, these mice can be bred as homozygotes. 
When using the Snca KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #016123 in your Materials and Methods section.