STOCK Nog^tm1.1Rmh/J

Stock number: 016117
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking the coding region of the noggin gene. This strain may have applications in studying bone morphogenetic protein signaling during embryonic development and in postnatal tissues.

Detailed description

In this strain loxP sites flank the coding region of the Noggin (Nog) gene. Homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. NOGGIN is a secreted protein involved in embryonic development including bone development, joint formation, and neural tube fusion. It also plays a role in neural induction, where it inhibits the bone morphogenetic protein (BMP)-signaling pathway. NOGGIN also inhibits Transforming growth factor (TGF)-β signal transduction by binding to TGF-β family ligands and preventing them from binding to their corresponding receptors. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will not express NOGGIN in the cre-expressing tissues. This strain may be useful for studying BMP-signaling during embryonic development and in postnatal tissues.

For example, when crossed to a strain expressing Cre recombinase in embryonic cells (see Stock No. 003376), this mutant mouse strain may be useful in studies of axial skeleton development.

Development

In this strain a loxP site was placed upstream of the coding region of the Noggin (Nog) gene, and a Frt-flanked neomycin resistance (neo) cassette and a second loxP site were placed downstream. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6;SJL-Tg(ACTFLPe)9205Dym/J mice (Stock No. 003800) to delete the neo cassette. The progeny were then crossed to remove the Flp-expressing transgene and the resulting Nog^fx/fx mice were backcrossed to C57BL/6J. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Nog^tm1.1Rmh
Name: targeted mutation 1.1, Richard M Harland
MGI accession ID: MGI:4941468
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Nog
Marker name: noggin
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Expressed genes: Nog,noggin,mouse, laboratory
Molecular note: A loxP site was placed upstream of the coding region of the Noggin (Nog) gene, and a FRT-flanked neomycin resistance (neo) cassette and a second loxP site were placed downstream. Flp-mediated recombination removed the neo cassette.