These floxed mutant mice possess loxP sites flanking the coding region of the noggin gene. This strain may have applications in studying bone morphogenetic protein signaling during embryonic development and in postnatal tissues.
Richard Harland, University of California, Berkeley
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Nog | noggin |
In this strain loxP sites flank the coding region of the Noggin (Nog) gene. Homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. NOGGIN is a secreted protein involved in embryonic development including bone development, joint formation, and neural tube fusion. It also plays a role in neural induction, where it inhibits the bone morphogenetic protein (BMP)-signaling pathway. NOGGIN also inhibits Transforming growth factor (TGF)-β signal transduction by binding to TGF-β family ligands and preventing them from binding to their corresponding receptors. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will not express NOGGIN in the cre-expressing tissues. This strain may be useful for studying BMP-signaling during embryonic development and in postnatal tissues.
For example, when crossed to a strain expressing Cre recombinase in embryonic cells (see Stock No. 003376), this mutant mouse strain may be useful in studies of axial skeleton development.
In this strain a loxP site was placed upstream of the coding region of the Noggin (Nog) gene, and a Frt-flanked neomycin resistance (neo) cassette and a second loxP site were placed downstream. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6;SJL-Tg(ACTFLPe)9205Dym/J mice (Stock No. 003800) to delete the neo cassette. The progeny were then crossed to remove the Flp-expressing transgene and the resulting Nogfx/fx mice were backcrossed to C57BL/6J. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | Nog, noggin, mouse, laboratory |
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Site of Expression |
Allele Name | targeted mutation 1.1, Richard M Harland |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Nogfx |
Gene Symbol and Name | Nog, noggin |
Gene Synonym(s) | |
Promoter | Nog, noggin, mouse, laboratory |
Expressed Gene | Nog, noggin, mouse, laboratory |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 11 |
Molecular Note | A loxP site was placed upstream of the coding region of the Noggin (Nog) gene, and a FRT-flanked neomycin resistance (neo) cassette and a second loxP site were placed downstream. Flp-mediated recombination removed the neo cassette. |
Mutations Made By | Richard Harland, University of California, Berkeley |
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Nogtm1.1Rmh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016117 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nog<tm1.1Rmh> |
Frozen Mouse Embryo | STOCK Nog<tm1.1Rmh>/J frozen embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Nog<tm1.1Rmh>/J frozen embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Nog<tm1.1Rmh>/J frozen embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Nog<tm1.1Rmh>/J frozen embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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