C.129P2(B6)-Git2^Gt(XG510)Byg/WeisJ

Stock number: 016095
Research areas: Cell Biology Research, Neurobiology Research, Reproductive Biology Research

Description

A targeting vector containing β-galactosidase was randomly inserted downstream of exon 2 of the endogenous G protein-coupled receptor kinase-interactor 2 (Git2) gene. These mice may be useful as a lacZ reporter for Git2 expression.

Detailed description

A targeting vector containing β-galactosidase was randomly inserted downstream of exon 2 of the endogenous G protein-coupled receptor kinase-interactor 2 (Git2) gene. Homozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This β-geo secretory trap mutation abolishes endogenous gene function and expresses a Git2-exon1-2/lacZ fusion protein. Git2 belongs to the family of ADP-ribosylation factor GTPase-activating proteins (ARF-GAP) and has been implicated in the regulation of G protein-coupled receptor sequestration, cell migration, T-cell activation, neuronal spine formation, and aggregate formation in Huntington's disease. GIT2 expression is seen throughout the liver, lungs, spleen, muscle, and the central nervous system. Expression of GIT2 is also seen during absent spermatid differentiation but is absent in mature spermatids. These mice may be useful as a lacZ reporter for Git2 expression.

Development

A "secretory trap" targeting vector containing β-galactosidase was electroporated into 129P2/OlaHsd-derived E14TG2a.4 embryonic stem (ES) cells. This vector inserted downstream of exon 2 of the endogenous G protein-coupled receptor kinase-interactor 2 (Git2) gene. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric mice were bred with C57BL/6 mice. These Git2(XG510) mice were backcrossed to BALB/c for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ (Stock No. 000651) for at least one generation to establish the colony.

Allele

Symbol: Git2^Gt(XG510)Byg
Name: gene trap XG510, BayGenomics
MGI accession ID: MGI:3762635
Generation method: Gene trapped
Attributes: Reporter
Synonyms: GIT2^-
Marker symbol: Git2
Marker name: G protein-coupled receptor kinase-interactor 2
Chromosome: 5
Strain of origin: 129P2/OlaHsd
Expressed genes: Bgeo,fusion of beta-galactosidase and neomycin phosphotransferase genes,E. coli
Site of expression: Git2 expression is seen throughout the liver, lungs, spleen, muscle, and the central nervous system. Expression of Git2 is also seen during spermatid differentiation but is absent in mature spermatids.
Molecular note: A gene trap vetor containing a beta-geo cassette was inserted into intron 2. Insertion was confirmed by PCR genotyping and expression of the lacZ gene was confirmed by beta-galactosidase staining. Two transcripts were detected by RT-PCR: one the expected fusion of exon 1 and 2 to the gene trap vector sequence and the other an alternative transcript lacking exon 2.