B6.129S4-Git1^{Gt(FHCRC-GT-S10-12C1)Sor}/WeisJ

Stock number: 016092
Research areas: Cell Biology Research, Developmental Biology Research, Neurobiology Research, Reproductive Biology Research, Research Tools

Description

A targeting vector containing β-galactosidase, a polyadenylation signal, and a PGK Hygromycin selection cassette, was randomly inserted downstream of exon 1 of the endogenous G protein-coupled receptor kinase-interactor 1 (Git1) gene. These mice may be useful as a lacZ reporter for Git1 expression.

Detailed description

A targeting vector containing β-galactosidase, a polyadenylation signal, and a PGK Hygromycin selection cassette, was randomly inserted downstream of exon 1 of the endogenous G protein-coupled receptor kinase-interactor 1 (Git1) gene. Heterozygous mice are viable, normal in size and do not display any gross physical or behavioral abnormalities, although some males have decreased fertility. Homozygous mice die within a few days after birth. This β-geo secretory trap mutation abolishes endogenous gene function and expresses a Git1-exon1/lacZ fusion protein. Git1 belongs to the family of ADP-ribosylation factor GTPase-activating proteins (ARF-GAP) and has been implicated in the regulation of G protein-coupled receptor sequestration, cell migration, neuronal spine formation, and aggregate formation in Huntington's disease. GIT1 expression is restricted to some areas of the brain, to cells lining blood vessels, bronchi, and bile ducts. Expression of GIT1 is absent in differentiating spermatids, but is present in mature spermatids and in mature ovarian follicles. These mice may be useful as a lacZ reporter for Git1 expression.

Development

A "secretory trap" targeting vector, containing β-galactosidase, a polyadenylation signal, and a PGK hygromycin selection cassette, was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. This vector inserted downstream of exon 1 of the endogenous G protein-coupled receptor kinase-interactor 1 (Git1) gene. Correctly targeted ES cells Correctly targeted ES cells (FHCRC-GT-S10-12C1) were injected into C57BL/6 blastocysts, and chimeric mice were bred with C57BL/6 mice. These Git1(S10-12C(P17-1B) mice were backcrossed to C57BL/6 for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Git1^{Gt(FHCRC-GT-S10-12C1)Sor}
Name: gene trap FHCRC-GT-S10-12C1, Philippe Soriano
MGI accession ID: MGI:3762634
Generation method: Gene trapped
Attributes: Reporter; Null/Knockout
Synonyms: GIT1^-
Marker symbol: Git1
Marker name: G protein-coupled receptor kinase-interactor 1
Marker synonyms: Cat-1; p95-APP1; p95Cat
Chromosome: 11
Strain of origin: 129S4/SvJaeSor
Site of expression: Git1 expression is restricted to some areas of the brain, to cells lining blood vessels, bronchi, and bile ducts. Expression of Git1 is absent in differentiating spermatids, but is present in mature spermatids and in mature ovarian follicles.
Molecular note: The gene trap vector containing a beta-geo cassette and a PGK-hygro cassette was inserted into intron 1. Insertion was confirmed by genomic PCR and expression of the lacZ cassette was confirmed by beta-galactosidase staining.