These lightning mice possess an ENU-induced point mutation (F503S) in the Ptprc gene and may be useful in studying the differential regulation of TCR signaling by altered CD45 expression levels.
Dr. Arthur Weiss, University of California, San Francisco
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | Ptprc | protein tyrosine phosphatase, receptor type, C |
These lightning mice possess an ENU-induced point mutation (F503S) in the protein tyrosine phosphatase, receptor type, C (Ptprc), gene. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Ptprc encodes CD45, a protein tyrosine kinase which is expressed in nucleated hematopoietic cells and is required for their activation. CD45 is also required for T cell receptor (TCR) signaling and T cell development. These mice contain only 15% of the normal level of CD45 expression on the surface of nucleated hematopoietic cells, without altering splicing of transcript isoforms. These mutant mice may be useful in studying the differential regulation of TCR signaling by altered CD45 expression levels.
Following multidose N-ethyl-N-nitrosourea (ENU) treatments to induce mutations in founder C57BL/6J mice, a genetic screen was utilized to identify lightning mice, exhibiting reduced expression of CD45 on the surface of all nucleated hematopoietic cells. Using a candidate gene approach a mutation was identified in the protein tyrosine phosphatase, receptor type, C (Ptprc), gene. Sequencing of Ptprc identified a T to C point mutation resulting in a phenylalanine to serine missense mutation (F503S). These mice were maintained on a coisogenic C57BL/6J background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | lightning |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | PtprcL |
Gene Symbol and Name | Ptprc, protein tyrosine phosphatase, receptor type, C |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 1 |
Molecular Note | ENU induced at T to C transition that results in the amino acid substitution of serine for phenylalanine at position 503 (F503S). Flow cytometry confirmed that protein half-life is reduced. |
Mutations Made By | Dr. Arthur Weiss, University of California, San Francisco |
When maintaining a live colony, homozygous mice may be bred together.
When using the C57BL/6-Ptprcltng/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016091 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ptprc<ltng> |
Frozen Mouse Embryo | C57BL/6-Ptprc<ltng>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Ptprc<ltng>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Ptprc<ltng>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Ptprc<ltng>/J | $3373.50 |
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