A targeting vector was designed to insert an internal ribosome entry site (IRES)-enhanced green fluorescent protein (eGFP) fusion protein, followed by a floxed neomycin (neo) resistance cassette, downstream of the endogenous translational stop codon of the interleukin 12b (Il12b) gene. These homozygous GET40 mice are viable, fertile, and normal in size. IL-12b, or p40, binds with p35 to form the heterodimeric cytokine IL-12, after induction by toll like receptors (TLRs), in macrophages and dendritic cells (DC). GET40 mice show p40 and GFP expression in DC in draining lymph nodes, after inoculation with Listeria monocytogenes or lipopolysaccharide (LPS), and promote Th1 differentiation. These mice may be useful for visualizing active IL-12 in DCs and macrophages during immune response.

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A targeting vector was designed to insert an internal ribosome entry site (IRES)-enhanced green fluorescent protein (eGFP) fusion protein, followed by a floxed neomycin (neo) resistance cassette, downstream of the endogenous translational stop codon of the interleukin 12b (Il12b) gene. These mice may be useful for visualizing active IL-12 in DCs and macrophages during immune response.

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