These Rims1 (regulating synaptic membrane exocytosis 1) floxed mice may be useful for generating animals lacking tissue-specific expression of the targeted gene.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
These mice possess loxP sites on either side of exon 6 in the Rims1 (regulating synaptic membrane exocytosis 1; Rim1α/β) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.
A targeting construct was designed to carry a loxP-flanked exon 6 into which a serine 413 to alanine (S413A) point mutation had been introduced. The floxed segment was immediately followed by an FRT-flanked drug resistance cassette including two copies of neomycin in intron 6. The construct was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. An ES cell clone in which the point mutation was repaired by a known DNA repair mechanism was selected to produce chimeric mice. The neomycin selection cassette was excised from chimeric animals though crosses with an Actb beta actin promoter Flp recombinase mouse originally made on a B6SJLF2 background, and possibly backcrossed to C57BL/6NCrl. This left exon 6 flanked by loxP sites. This strain was maintained on a predominantly C57BL/6 and 129 mixed background by the donating laboratory.
|Allele Name||targeted mutation 3, Thomas C Sudhof|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||fRIM1; RIM1alphabetafloxed|
|Gene Symbol and Name||Rims1, regulating synaptic membrane exocytosis 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A loxP site was inserted upstream of exon 6 and an frt-flanked double neo cassette with a 5' loxP site was inserted downstream of exon 6. The neo cassette was removed by germ line, flp mediated recombination leaving exon 6 floxed.|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the R1F-RIM1alpha/beta floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #015832 in your Materials and Methods section.