When crossed with a Cre recombinase-expressing strain, this floxed mutant strain is useful in eliminating tissue-specific expression of the Erc2 (ELKS/RAB6-interacting/CAST family member 2) gene.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
A 5'UTR exon and the first coding exon of the Erc2 (ELKS/RAB6-interacting/CAST family member 2; ELKS2, CAST1) gene are flanked by loxP sites in these targeted mutant mice. An in-frame tetracysteine tag is located in exon 1. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Normal expression of the targeted gene is demonstrated by the floxed allele.
Widespread cre excision of the floxed exons blocks expression (see Stock No. 008391).
A targeting vector was designed to flank a 5'UTR exon, the first coding exon (with inserted in-frame tetracysteine tag), and an FRT-flanked neomycin cassette with loxP sites. The vector was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. The neomycin selection cassette was excised from chimeric animals though crosses with an Actb beta actin promoter Flp recombinase mouse originally made on a B6SJLF2 background, and possibly backcrossed to C57BL/6NCrl. This strain was maintained on a predominantly C57BL/6 and 129 mixed background by the donating laboratory.
|Allele Name||targeted mutation 1.2, Thomas C Sudhof|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||E2F-ELKS2 floxed; ELKS2alphaf|
|Gene Symbol and Name||Erc2, ELKS/RAB6-interacting/CAST family member 2|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A targeting vector was designed to flank a 5'UTR exon, the first coding exon (with inserted in-frame tetracysteine tag), and an FRT-flanked neomycin cassette with loxP sites. Flp-mediated recombination removed the neo cassette.|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
When maintained as a live colony, homozygotes may be bred.
When using the E2F-ELKS2 floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #015831 in your Materials and Methods section.