These spH64 transgenic mice express the fluorescent indicator synaptopHluorin in many regions of the brain and may be useful in studies of synaptic vesicle recycling.
Venkatesh N. Murthy, Harvard University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These transgenic mice express spH, the pH-sensitive variant of the enhanced green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter. In founder line spH64, transgene expression is detected in many regions of the brain. spH expression is detected mainly in GABAergic or inhibitory synaptic terminals of dissociated hippocampal neurons. Synaptic activity, as determined by fluorescence, is detectable starting at approximately 10 days in vitro in cultured hippocampal neurons.
A transgenic construct containing spH, the pH-sensitive variant of the enhanced green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter was injected into fertilized B6CBAF1 mouse eggs. Founder line spH64 was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least twice to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | Vamp2, vesicle-associated membrane protein 2, mouse, laboratory |
Site of Expression | pH-sensitive GFP is expressed in in GABAergic or inhibitory synaptic terminals of dissociated hippocampal neurons. |
Allele Name | transgene insertion 64, Venkatesh N Murthy |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | spH64 |
Gene Symbol and Name | Tg(Thy1-spH)64Vnmu, transgene insertion 64, Venkatesh N Murthy |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Vamp2, vesicle-associated membrane protein 2, mouse, laboratory |
Site of Expression | pH-sensitive GFP is expressed in in GABAergic or inhibitory synaptic terminals of dissociated hippocampal neurons. |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
Molecular Note | The transgenic construct contains spH, the pH-sensitive variant of the green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter. In founder line spH64, transgene expression is detected in GABAergic or inhibitory neurons of the hippocampus. Synaptic activity, as determined by fluorescence, is detectable starting at approximately postnatal day 10 in cultured hippocampal glutamatergic neurons. |
Mutations Made By | Venkatesh Murthy, Harvard University |
When maintaining a live colony, these mice can be bred as hemizygotes.
When using the B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #015814 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Thy1-spH)64Vnmu |
Frozen Mouse Embryo | B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ Frozen Embryo | $3373.50 |
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