B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ

Stock number: 015814
Research areas: Neurobiology Research, Research Tools

Description

These spH64 transgenic mice express the fluorescent indicator synaptopHluorin in many regions of the brain and may be useful in studies of synaptic vesicle recycling.

Detailed description

These transgenic mice express spH, the pH-sensitive variant of the enhanced green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter. In founder line spH64, transgene expression is detected in many regions of the brain. spH expression is detected mainly in GABAergic or inhibitory synaptic terminals of dissociated hippocampal neurons. Synaptic activity, as determined by fluorescence, is detectable starting at approximately 10 days in vitro in cultured hippocampal neurons.

Development

A transgenic construct containing spH, the pH-sensitive variant of the enhanced green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter was injected into fertilized B6CBAF1 mouse eggs. Founder line spH64 was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least twice to establish the colony.

Allele

Symbol: Tg(Thy1-spH)64Vnmu
Name: transgene insertion 64, Venkatesh N Murthy
MGI accession ID: MGI:4949114
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: spH64
Marker symbol: Tg(Thy1-spH)64Vnmu
Marker name: transgene insertion 64, Venkatesh N Murthy
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: Vamp2,vesicle-associated membrane protein 2,mouse, laboratory; GFP,Green Fluorescent Protein,
Site of expression: pH-sensitive GFP is expressed in in GABAergic or inhibitory synaptic terminals of dissociated hippocampal neurons.
Molecular note: The transgenic construct contains spH, the pH-sensitive variant of the green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter. In founder line spH64, transgene expression is detected in GABAergic or inhibitory neurons of the hippocampus. Synaptic activity, as determined by fluorescence, is detectable starting at approximately postnatal day 10 in cultured hippocampal glutamatergic neurons.