The cTVA transgene contains the human ubiquitin C (UBC) promoter driving expression of a loxP-flanked destabilized green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. These cTVA mice may be useful for gene delivery via infection with ASLV-derived gene vectors. Although GFP expression in UBC-expressing cells was anticipated, none is detected.
Connie Cepko, Harvard Medical School, HHMI
Genetic Background | Generation |
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Allele Type |
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Transgenic (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) |
UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins.
The cTVA transgene in this strain carries the human ubiquitin C (UBC) promoter/enhancer elements driving expression of a loxP-flanked destabilized green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile.
Although GFP expression in UBC-expressing cells was anticipated, none is detected. This may be associated with the use of destabilized GFP and/or low expression levels.
Cre-mediated excision of the floxed-GFP-STOP cassette results in TVA overexpression in UBC-expressing cells. Upon expression of TVA, these cells are capable of binding viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These cTVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV-A glycoproteins.
The cTVA transgene was designed with the human ubiquitin C (UBC) promoter driving expression of a loxP-flanked destabilized green fluorescent protein (GFP containing its own Stop sequence) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. The transgene was microinjected into fertilized C57BL/6 X SJL/J F2 oocytes, and mice from founder line 2 were bred to C57BL/6J mice for at least ten generations. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | TVA, subgroup A avian leukosis virus receptor, quail |
Site of Expression | GFP expression is seen in all tissues. Cre excision of the floxed-GFP/STOP cassette results in retroviral receptor (TVA) gene expression. |
Allele Name | transgene insertion 2, Constance L Cepko |
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Allele Type | Transgenic (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) |
Allele Synonym(s) | cTVA line 2 |
Gene Symbol and Name | Tg(UBC-GFP,-TVA)2Clc, transgene insertion 2, Constance L Cepko |
Gene Synonym(s) | |
Promoter | UBC, ubiquitin C, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | TVA, subgroup A avian leukosis virus receptor, quail |
Site of Expression | GFP expression is seen in all tissues. Cre excision of the floxed-GFP/STOP cassette results in retroviral receptor (TVA) gene expression. |
Strain of Origin | (C57BL/6 x SJL/J)F2 |
Chromosome | UN |
Molecular Note | The cTVA transgene was designed with the human ubiquitin C (UBC) promoter driving expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. |
Mutations Made By | Connie Cepko, Harvard Medical School, HHMI |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the B6.Cg-Tg(UBC-GFP,-TVA)2Clc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #015806 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(UBC-GFP,-TVA)2Clc |
Frozen Mouse Embryo | B6.Cg-Tg(UBC-GFP -TVA)2Clc/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(UBC-GFP -TVA)2Clc/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(UBC-GFP -TVA)2Clc/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(UBC-GFP -TVA)2Clc/J | $3373.50 |
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