| Allele Name | transgene insertion 21, Venkatesh N Murthy |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | spH21 |
| Gene Symbol and Name | Tg(Thy1-spH)21Vnmu, transgene insertion 21, Venkatesh N Murthy |
| Gene Synonym(s) | |
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
|---|
| Expressed Gene | GFP, Green Fluorescent Protein, |
|---|
| Expressed Gene | Vamp2, vesicle-associated membrane protein 2, mouse, laboratory |
|---|
| Site of Expression | pH-sensitive GFP is expressed in glutamatergic or excitatory neurons of many brain regions, including the cortex and the hippocampus. |
|---|
| Strain of Origin | (C57BL/6 x CBA)F1 |
| Chromosome | UN |
| Molecular Note | The transgenic construct contains spH, the pH-sensitive variant of the green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter. In founder line spH21, transgene expression is detected in glutamatergic or excitatory neurons of the hippocampus. Synaptic activity, as determined by fluorescence, is detectable starting at approximately postnatal day 10 in cultured hippocampal glutamatergic neurons. |
| Mutations Made By | Venkatesh Murthy, Harvard University |
