These spH21 transgenic mice express fluorescent indicator synaptopHluorin in many regions of the brain and may be useful in studies of synaptic vesicle recycling.
Venkatesh N. Murthy, Harvard University
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter) |
These transgenic mice express spH, the pH-sensitive variant of the green fluorescent protein (superecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter. In founder line spH21, transgene expression is detected in glutamatergic or excitatory neurons of many brain regions, including the cortex and the hippocampus. Synaptic activity, as determined by fluorescence, is detectable starting at approximately 14 days in vitro in cultured hippocampal neurons.
A transgenic construct containing spH, the pH-sensitive variant of the green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter was injected into fertilized B6CBAF1 mouse eggs. Founder line spH21 was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Expressed Gene | Vamp2, vesicle-associated membrane protein 2, mouse, laboratory |
Site of Expression | pH-sensitive GFP is expressed in glutamatergic or excitatory neurons of many brain regions, including the cortex and the hippocampus. |
Allele Name | transgene insertion 21, Venkatesh N Murthy |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | spH21 |
Gene Symbol and Name | Tg(Thy1-spH)21Vnmu, transgene insertion 21, Venkatesh N Murthy |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Vamp2, vesicle-associated membrane protein 2, mouse, laboratory |
Site of Expression | pH-sensitive GFP is expressed in glutamatergic or excitatory neurons of many brain regions, including the cortex and the hippocampus. |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
Molecular Note | The transgenic construct contains spH, the pH-sensitive variant of the green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter. In founder line spH21, transgene expression is detected in glutamatergic or excitatory neurons of the hippocampus. Synaptic activity, as determined by fluorescence, is detectable starting at approximately postnatal day 10 in cultured hippocampal glutamatergic neurons. |
Mutations Made By | Venkatesh Murthy, Harvard University |
When maintaining a live colony, these mice can be bred as hemizygotes.
When using the B6;CBA-Tg(Thy1-spH)21Vnmu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014651 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemozygous or Non carrier for Tg(Thy1-spH)21Vnmu |
Frozen Mouse Embryo | B6;CBA-Tg(Thy1-spH)21Vnmu/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(Thy1-spH)21Vnmu/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(Thy1-spH)21Vnmu/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;CBA-Tg(Thy1-spH)21Vnmu/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.