B6;CBA-Tg(Thy1-spH)21Vnmu/J

Stock number: 014651
Research areas: Neurobiology Research, Research Tools

Description

These spH21 transgenic mice express fluorescent indicator synaptopHluorin in many regions of the brain and may be useful in studies of synaptic vesicle recycling.

Detailed description

These transgenic mice express spH, the pH-sensitive variant of the green fluorescent protein (superecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter. In founder line spH21, transgene expression is detected in glutamatergic or excitatory neurons of many brain regions, including the cortex and the hippocampus. Synaptic activity, as determined by fluorescence, is detectable starting at approximately 14 days in vitro in cultured hippocampal neurons.

Development

A transgenic construct containing spH, the pH-sensitive variant of the green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter was injected into fertilized B6CBAF1 mouse eggs. Founder line spH21 was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Thy1-spH)21Vnmu
Name: transgene insertion 21, Venkatesh N Murthy
MGI accession ID: MGI:4882125
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Thy1-spH)21Vnmu
Marker name: transgene insertion 21, Venkatesh N Murthy
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: Vamp2,vesicle-associated membrane protein 2,mouse, laboratory; GFP,Green Fluorescent Protein,
Site of expression: pH-sensitive GFP is expressed in glutamatergic or excitatory neurons of many brain regions, including the cortex and the hippocampus.
Molecular note: The transgenic construct contains spH, the pH-sensitive variant of the green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter. In founder line spH21, transgene expression is detected in glutamatergic or excitatory neurons of the hippocampus. Synaptic activity, as determined by fluorescence, is detectable starting at approximately postnatal day 10 in cultured hippocampal glutamatergic neurons.