These floxed YY1 transcription factor (Yy1tm2Yshi) mice may be useful in generating conditional mutations for studies of cell proliferation, apoptosis, and differentiation.
Yang Shi, Children's Hospital Boston
These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue(s).
When crossed with mice that express Cre recombinase specifically in early B-cell progenitors, this mutant mouse strain may be useful in studies of B-cell maturation.
A targeting vector containing a FRT site flanked PGK-Neo cassette and a loxP site was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 1 of the targeted gene, and another loxP site was inserted upstream of exon 1. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice expressing Flpe recombinase, on the 129S4/SvJaeSor background (Stock No. 003946). The donating investigator reported that the mice that retained the loxP site flanked exon 1, promoter and first intron, were then bred to C57BL/6 mice for 4 generations to remove the Flpe recombinase allele (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. At least 3 markers are segregating. Also, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Allele Name||targeted mutation 2, Yang Shi|
|Allele Type||Targeted (Conditional ready (e.g. floxed), Hypomorph)|
|Gene Symbol and Name||Yy1, YY1 transcription factor|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Exon 1 and the promoter region were flanked with loxP sites by homologous recombination. An FRT-flanked neomycin cassette was inserted downstream of the 3' loxP site. Protein expression in heterozygotes is reduced by about 25% compared to wild-type mice.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129S4-Yy1tm2Yshi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014649 in your Materials and Methods section.