C57BL/6-Tg(Cd4-TcraDN32D3)1Aben/J

Stock number: 014639
Product name: Vα14-Jα18 transgenic
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Vα14-Jα18 transgenic mice contain CD4 promoter/enhancer elements driving expression of prearranged Vα14-Jα18 T cell receptor (TCR) cDNA from hybridoma line DN32.D3. These mice may be useful for studying positive/negative selection, T cell receptor interactions, and NKT cell function.

Detailed description

Vα14-Jα18 transgenic mice contain CD4 promoter/enhancer elements driving expression of prearranged Vα14-Jα18 T cell receptor (TCR) cDNA from hybridoma line DN32.D3. Hemizygous mice are viable and fertile. These mice overexpress Natural Killer T (NKT) cells, or Vα14 NKT cells, in the liver, thymus, spleen, and lymph nodes. These mice may be useful for studying positive/negative selection, T cell receptor interactions, and NKT cell function.

Development

The Vα14-Jα18 transgene was developed containing prearranged Vα14-Jα18 T cell receptor (TCR) cDNA (DN32.D3 hybridoma line) downstream of CD4 promoter/enhancer elements. The construct was injected into fertilized C57BL/6 oocytes. Germ line transmission of the transgene was confirmed and founder mice were backcrossed to C57BL/6 mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Cd4-TcraDN32D3)1Aben
Name: transgene insertion 1, Albert Bendelac
MGI accession ID: MGI:4880641
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: Tg-Valpha18-Jalpha18
Marker symbol: Tg(Cd4-TcraDN32D3)1Aben
Marker name: transgene insertion 1, Albert Bendelac
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory
Molecular note: The Valpha14-Jalpha18 transgene was developed containing prearranged Valpha14-Jalpha18 T cell receptor (TCR) cDNA (DN32.D3 hybridoma line) downstream of CD4 promoter/enhancer elements. Line 1 was generated.