C;129S4-Rag2^tm1.1Flv Il2rg^tm1.1Flv/J

Stock number: 014593
Product name: Rag2- γc-
Strain synonyms: BRG
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Rag2/Il2rg compound mutant mice (also called Rag2^-/- γc^-/- mice) exhibit T cell, B cell and NK cell immunodeficiencies that make them effective transplant hosts for human immune cells.

Detailed description

Both the Rag2 and the X-linked Il2rg genes were targeted for deletion in these compound mutant mice. Homozygous Rag2 and hemizygous male/homozygous female Il2rg animals lack T, B, and NK cells. Their immunodeficiencies make them particularly useful as transplant hosts. Liver hematopoietic stem and progenitor cell transplants create a humanized model that may be used to develop and test novel immune therapeutic and preventative strategies.

The creation of this strain was funded by the Bill and Melinda Gates Foundation.

Development

V17 embryonic stem (ES) cells (derived from BALB/cTac x 129S4/SvJae mice) were used to sequentially delete the coding regions of the Rag2 and X chromosome-linked Il2rg genes. A loxP-flanked PGK-neomycin cassette was targeted to Rag2 and a loxP-flanked human ubiquitin promoter-driven hygromycin cassette was directed to Il2rg. Transient infection with a cre plasmid excised the floxed segments. Chimeras were crossed once to BALB/c and the mice were intercrossed to produce mice that are homozygous for the Rag2 mutation and hemizygous/homozygous for the Il2rg mutation.

Allele

Symbol: Rag2^tm1.1Flv
Name: targeted mutation 1.1, Richard A Flavell
MGI accession ID: MGI:4887836
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Rag2
Marker name: recombination activating gene 2
Chromosome: 2
Strain of origin: BALB/cAnNTac x 129S4/SvJae
Molecular note: A floxed PGK-neomycin cassette replaced the coding region. Cre-mediated recombination removed the neo cassette.
General note: Originally generated in conjunction with Il2rg^tm1.1Flv.

Allele

Symbol: Il2rg^tm1.1Flv
Name: targeted mutation 1.1, Richard A Flavell
MGI accession ID: MGI:4887837
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il2rg
Marker name: interleukin 2 receptor, gamma chain
Chromosome: X
Strain of origin: BALB/cAnNTac x 129S4/SvJae
Molecular note: A floxed human ubiquitin promoter-driven hygromycin cassette replaced the coding region. Cre-mediated recombination removed the hygro cassette.
General note: Originally generated in conjunction with Rag2^tm1.1Flv.