Overview

Also Known As:hHGFki;scid

Of note, hHGFki;scid mice also harboring the Il2Rγc^null mutation on the NOD-congenic background are available: NSG-hHGFki (Stock No. 014553).

These hHGFki;scid mice harbor the "humanized" knock-in mutation (hHGFki) that replaces the mouse hepatocyte growth factor (HGF) coding factor, and the Prkdc^scid mutation that results in T- and B-cell deficiency. These mice may be useful in studying the HGF/MET pathway in human tumor xenografts and mouse tumor allografts.

Donating Investigator

William M Rideout III, AVEO Pharmaceuticals, Inc

Genetic overview

Genetic Background	Generation

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

Hgf^{tm1.1(HGF)Aveo}

Allele Type	Gene Symbol	Gene Name
Targeted (Inserted expressed sequence, Humanized sequence)	Hgf	hepatocyte growth factor

View Genetics

Research Applications

Cell Biology Research
Research Tools
Internal/Organ Research
Immunology, Inflammation and Autoimmunity Research
Virology Research
Cancer Research
Hematological Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Important Note

Of note, hHGFki;scid mice also harboring the Il2Rγc^null mutation on the NOD-congenic background are available: NSG-hHGFki (Stock No. 014553).

Detailed Description

Mice homozygous for both the hHGFki and Prkdc^scid alleles, also called immunocompromised hHGFki mice, are viable and fertile.

The hHGFki allele is a "humanized" knock-in mutation that replaces the mouse hepatocyte growth factor (HGF) coding region downstream of the signal sequence with the human HGF cDNA sequence. As a result, the endogenous mouse promoter drives expression of human HGF. While the human HGF activates both the human and murine form of its tyrosine kinase receptor (met proto-oncogene (MET; c-Met)), the murine HGF is unable to activate human MET. Mice homozygous for hHGFki express only the human form of HGF. In homozygous hHGFki mice, HGF expression from the knock-in allele is observed in developing embryo, as well as adult liver, kidney and lung. hHGFki homozygous mice exhibit an increase in serum HGF after clotting.

Mice homozygous for the Prkdc^scid mutation exhibit T- and B-cell deficiency.

Development

A targeting vector was designed to replace exons 3-6 of the mouse hepatocyte growth factor (Hgf) locus with a cDNA sequence encoding exons 2-18 of the human hepatocyte growth factor (HGF), a polyA signal, and a frt-flanked PGK-neo cassette. The construct was electroporated into 129S6/SvEvTac-derived JB26A10 embryonic stem (ES) cells. Next, ES cells were transiently transfected with a FLPe expressing plasmid to remove the frt-flanked PGK-neo cassette. Correctly targeted ES cells were then injected into recipient blastocysts and chimeric mice were bred with C57BL/6 mice to establish the hHGFki colony. The hHGFki mice were then bred with C3H^scid (C3SnSmn.CB17-Prkdc^scid/J ; see Stock No. 001131) to generate the hHGFki;scid double mutant mice. Mice homozygous for both alleles were bred together for many generations prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred together and/or with C3SnSmn.CB17-Prkdc^scid/J ; see Stock No. 001131) for at least one generation to establish the colony. After this, mice homozygous for both alleles were bred together to maintain the colony.

Expression Data

Expressed Gene	HGF, hepatocyte growth factor, human
Site of Expression

Control Suggestions

Approximate Controls

Additional Information

Considerations for Choosing Controls

Genetics

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	SCID
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)
Site of Expression	T and B lymphocytes.
Strain of Origin	C.BKa-Igh^b/Icr
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Hgf^{tm1.1(HGF)Aveo}

Allele Symbol: Hgf^{tm1.1(HGF)Aveo}

Allele Name	targeted mutation 1.1, AVEO Pharmaceuticals Inc
Allele Type	Targeted (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	hHGFki
Gene Symbol and Name	Hgf, hepatocyte growth factor
Gene Synonym(s)
Expressed Gene	HGF, hepatocyte growth factor, human
Strain of Origin	129S6/SvEvTac
Chromosome	5
General Note	ES cells = JB26A10 (129S6/SvEvTac)
Molecular Note	A targeting vector was designed to replace exons 3-6 of the mouse hepatocyte growth factor (Hgf) locus with a cDNA sequence encoding exons 2-18 of the human hepatocyte growth factor (HGF), a polyA signal, and a frt-flanked PGK-neo cassette. Flp-mediated recombination removed the neo cassette.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Cell Biology Research
- Signal Transduction
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B cell deficiency
  - T cell deficiency
  - B and T cell deficiency
- Inflammation
  - B and T cell deficiency
- Lymphoid Tissue Defects
  - B and T cell deficiency
- Intracellular Signaling Molecules
- Autoimmunity
  - B and T cell deficiency
  - B cell deficiency
- Growth Factors/Receptors/Cytokines
- T Cell Receptor Signaling Defects
  - B and T cell deficiency, xenograft/transplant host
  - B and T cell deficiency
Cancer Research
- Other
  - tumor metastasis
- Toxicology
  - B and T cell deficiency, xenograft/transplant host
  - xenograft/transplant host
- Growth Factors/Receptors/Cytokines
Research Tools
- Toxicology Research
  - xenograft/transplant host
  - B and T cell deficiency, xenograft transplant host
- Immunology, Inflammation and Autoimmunity Research
  - T cell deficiency
  - T cell deficiency, xenograft/transplant host
  - B and T cell deficiency
  - B cell deficiency
- Cancer Research
  - xenograft/transplant host
  - B and T cell deficiency, xenograft/transplant host
  - B cell deficiency
  - T cell deficiency
Hematological Research
- Immunological Defects
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
  - T cell deficiency
- Adrenal Cortex Defects
  - T cell deficiency
- Thymus Defects
  - B and T cell deficient

Genotype: Prkdc^scid related

Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
  - AIDS research tool

Mammalian Phenotype Terms by Genotype

References

Additional - Hgf^{tm1.1(HGF)Aveo} related

Additional - Prkdc^scid related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- End Point Analysis:Prkdc End Point
- Standard PCR:Hgf
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, mice homozygous for the hHGFki allele and homozygous for the Prkdc^scid allele may be bred together. There are no special husbandry requirements other than those necessary for immunocompromised Prkdc^scid mice.
- Additional Breeding and Husbandry Support
Mating System
- See "Breeding Considerations"
Citation
When using the hHGFki;scid mouse strain in a publication, please cite the originating article(s) and include JAX stock #014543 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service/Product	Description	Price
> >	Heterozygous for Hgf<tm1.1(HGF)Aveo>, Homozygous for Prkdc<scid>. Minimum of 1 pair provided

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Use of MICE only available to non-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:hHGFki;scid

Donating Investigator

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Prkdcscid

Allele Symbol: Prkdcscid

Hgftm1.1(HGF)Aveo

Allele Symbol: Hgftm1.1(HGF)Aveo

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdcscid related

Mammalian Phenotype Terms by Genotype

References

Additional - Hgftm1.1(HGF)Aveo related

Additional - Prkdcscid related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Not-For-Profit & Academic Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Prkdc^scid

Allele Symbol: Prkdc^scid

Hgf^{tm1.1(HGF)Aveo}

Allele Symbol: Hgf^{tm1.1(HGF)Aveo}

Genotype: Prkdc^scid related

Additional - Hgf^{tm1.1(HGF)Aveo} related

Additional - Prkdc^scid related