STOCK Hey2^tm1Eno/J

Stock number: 014178
Research areas: Research Tools

Description

These Hrt2^loxP floxed mutant mice may be useful in generating conditional mutations for studying cardiac development.

Detailed description

These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene, which encode amino acids 29-82. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in vascular smooth muscle (see Stock No. 004746 for example), this mutant mouse strain may be useful in studies of ventricular septal defect cardiac development.

When bred to a strain with Cre recombinase expression in developing heart, pharynx, this mutant mouse strain may be useful in studies of cardiac development.

Development

A targeting vector containing a FRT-flanked neomycin resistance cassette and a single loxP site was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into 129S6/SvEvTac derived SM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to hACTB::FLPe transgenic mice (expressing FLPe recombinase under the control of the beta actin promoter) to remove the selection cassette. Mice that retained the loxP site flanked exons 2 and 3 were then bred to C57BL/6 mice to remove the FLPe transgene. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Hey2^tm1Eno
Name: targeted mutation 1, Eric N Olson
MGI accession ID: MGI:3711158
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Hey2
Marker name: hairy/enhancer-of-split related with YRPW motif 2
Chromosome: 10
Strain of origin: 129S6/SvEvTac
Molecular note: LoxP sites were introduced to flank exons 1-3. A neo included in the vector was removed by flp mediated recombination.