NOD.NOR-Idd4^NOR/LtJ/JsdDvsJ

Stock number: 014164
Product name: NOD.NOR-c11
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

This Idd4 congenic strain is the result of crossing a Chromosome 11 genomic segment from NOR/LtJ onto the NOD/Jsd background. This strain experiences significantly reduced diabetes and may be useful to identify genes in the Idd4 region associated with diabetes resistance. As Cyclophosphamide treated females are resistant to diabetes, this strain may also be useful for studying the sexual dimorphism of type 1 diabetes.

Detailed description

Mice from this NOD/Jsd congenic strain carry chromosome 11 alleles derived from strain NOR/LtJ at the insulin dependent diabetes susceptibility 4 (Idd4) loci. The Idd4 region contains candidate genes Psmb6, Pld2, Alox15, Alox12e and Cxcl16. Diabetes onset and incidence, among NOD-Idd4^NOR/LtJ congenic mice, is significantly later and reduced, with less than 30% of the females becoming diabetic by 30 weeks of age. Cyclophosphamide treated NOD-Idd4^NOR/LtJ females, but not males, are resistant to diabetes.
This strain may be useful for studying sexual dimorphism of type 1 diabetes; identifying candidate genes associated with diabetes resistance and positionally cloning the Idd4 resistant loci.

Development

This strain was produced by mating mice from NOD/Jsd (NOD) to NOR/LtJ (Stock No. 2050) followed by 16 additional marker assisted crosses to NOD with selection for chromosome 11, Idd4 markers. Siblings carrying the NOR/LtJ derived segments denoted by markers D11Mit310 (53.9Mb) through D11Bhm149 (77.4Mb) were intercrossed to make homozygous. In 2010, the Type 1 Diabetes Resource received this strain at generation N17F6.

Allele

Symbol: Idd4^NOR/LtJ
Name: NOR/LtJ
MGI accession ID: MGI:3582442
Generation method: QTL
Marker symbol: Idd4
Marker name: insulin dependent diabetes susceptibility 4
Chromosome: 11
Strain of origin: NOR/LtJ
Molecular note: This allele confers resistance to cyclophosphamide-induced insulitis in female animals compared to NOD/Jsd. This allele also confers resistance to spontaneous development of insulin dependent diabetes compared to NOD/Jsd.