A1R knock-out has a neomycin resistance cassette replacing exon 2 of the endogenous adenosine A1 receptor gene. These mice may be useful for studying pharmacological uses of adenosine regarding cardiac function, oxygen consumption, and insulin secretion.
Bertil Fredholm, Karolinjska Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Adora1 | adenosine A1 receptor |
In this A1R strain, neomycin resistance (neo) cassette replaces exon 2 of the endogenous adenosine A1 receptor (Adora1) gene, abolishing gene function. Heterozygotes are viable, fertile, and normal in size. Homozygotes have a decreased survival rate after 5 months of age and males exhibit reduced fertility. The binding of adenosine to A1R inhibits excitatory synaptic transmissions in the brain and causes dilation of veins and arteries. These mice exhibit an increase in hyperalgesia, anxiety, hypoxic damage, lipolysis, insulin secretion, osteopetrosis, and susceptibility to epilepsy. They also have abolished tubuloglomerular feedback and a reduction in ischemic preconditioning. These mice have a decrease recovery rate after hypoxic or ischemic stress.
A targeting vector was designed to replace exon 2 of the adenosine A1 receptor (Adora1 or A1R) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6N females to generate a colony of A1R mice. These mice were backcrossed on to a C57BL/6NTac background using a speed congenic protocol at The Jackson Laboratory. Mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Bertil B Fredholm |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | A1AR-; A1-KO; A1R- |
Gene Symbol and Name | Adora1, adenosine A1 receptor |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 1 |
Molecular Note | A PGK-neo cassette replaced most of the coding region and a portion of the of the 3' flanking region of the targeted exon. An absence of the normal protein was observed using in situ hybridization and autoradiographic analysis using a labeled ligand, DPCPX. |
Mutations Made By | Bertil Fredholm, Karolinjska Institute |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6NJ inbred mice (Stock No. 005304). Homozygotes have a decreased survival rate after 5 months of age and males exhibit reduced fertility.
When using the A1R knock-out (A1R KO) mouse strain in a publication, please cite the originating article(s) and include JAX stock #014161 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Adora1<tm1Bbf> |
Frozen Mouse Embryo | B6N.129P2-Adora1<tm1Bbf>/J | $2595.00 |
Frozen Mouse Embryo | B6N.129P2-Adora1<tm1Bbf>/J | $2595.00 |
Frozen Mouse Embryo | B6N.129P2-Adora1<tm1Bbf>/J | $3373.50 |
Frozen Mouse Embryo | B6N.129P2-Adora1<tm1Bbf>/J | $3373.50 |
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